Lanzer M, de Bruin D, Ravetch J V
DeWitt Wallace Research Laboratory, Division of Molecular Biology, Sloan-Kettering Institute, New York, NY 10021.
Nucleic Acids Res. 1992 Jun 25;20(12):3051-6. doi: 10.1093/nar/20.12.3051.
The Plasmodium falciparum gene encoding the knob associated histidine-rich protein (KAHRP) is shown to be transcriptionally regulated during its expression in the intraerythrocytic cycle as demonstrated by stage specific nuclear run-on analysis. The genomic organization of the KAHRP gene was determined and the structural basis for the stage specific transcription investigated. A sequence motif with two-fold symmetry was found 160 bp upstream of the RNA initiation site. This sequence element interacts with parasite derived nuclear extracts in a stage specific manner that correlates with the transcriptional activity of the KAHRP gene. These studies suggest a functional role for this structural element in the developmental regulation of a P. falciparum erythrocytic gene.
编码富含组氨酸的 knobs 相关蛋白（KAHRP）的恶性疟原虫基因，经阶段特异性核延伸分析表明，在其红细胞内周期表达过程中受到转录调控。确定了 KAHRP 基因的基因组结构，并研究了阶段特异性转录的结构基础。在 RNA 起始位点上游 160 bp 处发现了一个具有双重对称性的序列基序。该序列元件以阶段特异性方式与寄生虫来源的核提取物相互作用，这与 KAHRP 基因的转录活性相关。这些研究表明该结构元件在恶性疟原虫红细胞基因的发育调控中具有功能作用。
