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由RecA蛋白与寡核苷酸和双链DNA形成的接头会阻碍转录的起始和延伸。

Joints formed by RecA protein from oligonucleotides and duplex DNA block initiation and elongation of transcription.

作者信息

Golub E I, Ward D C, Radding C M

机构信息

Department of Genetics, Yale University School of Medicine, New Haven, CT 06510.

出版信息

Nucleic Acids Res. 1992 Jun 25;20(12):3121-5. doi: 10.1093/nar/20.12.3121.

DOI:10.1093/nar/20.12.3121
PMID:1620609
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC312447/
Abstract

In the presence of the non-hydrolyzable analog of ATP, ATP gamma S, RecA protein can polymerize on an oligodeoxy-ribonucleotide to form a stable oligonucleoprotein filament that can find its homologous sequence in double-stranded DNA. The homologous joint formed by the oligonucleotide and duplex DNA is stable only if RecA protein is not removed. Such a nucleoprotein joint, covering a part or all of the promoter region of T3 or T7 phage RNA polymerase, blocked transcription directed by those polymerases. The same kind of joint, located downstream of the RNA polymerase promoter, also inhibited elongation of transcription and caused accumulation of truncated transcripts. These observations suggest that RecA protein can be used to shut off transcription from any promoter of known sequence.

摘要

在存在ATP的不可水解类似物ATPγS的情况下，RecA蛋白可以在寡聚脱氧核糖核苷酸上聚合，形成稳定的寡核苷酸蛋白细丝，该细丝能够在双链DNA中找到其同源序列。仅当RecA蛋白不被去除时，由寡核苷酸和双链DNA形成的同源接头才是稳定的。这种覆盖T3或T7噬菌体RNA聚合酶启动子区域一部分或全部的核蛋白接头，阻断了那些聚合酶指导的转录。位于RNA聚合酶启动子下游的同类型接头，也抑制转录延伸并导致截短转录本的积累。这些观察结果表明，RecA蛋白可用于关闭来自任何已知序列启动子的转录。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/013b/312447/83cc09e68ba3/nar00086-0187-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/013b/312447/d14946f48de6/nar00086-0187-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/013b/312447/ddbe343b01f8/nar00086-0187-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/013b/312447/0b36ec1946aa/nar00086-0187-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/013b/312447/83cc09e68ba3/nar00086-0187-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/013b/312447/d14946f48de6/nar00086-0187-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/013b/312447/ddbe343b01f8/nar00086-0187-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/013b/312447/0b36ec1946aa/nar00086-0187-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/013b/312447/83cc09e68ba3/nar00086-0187-d.jpg

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本文引用的文献

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Homologous pairing in genetic recombination. Purification and characterization of Escherichia coli recA protein.遗传重组中的同源配对。大肠杆菌recA蛋白的纯化与特性分析。
J Biol Chem. 1981 Jul 25;256(14):7557-64.
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Role of RecA protein spiral filaments in genetic recombination.RecA蛋白螺旋丝在基因重组中的作用。
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Letter: The structure of polydeoxyguanylic acid with polydeoxycytidylic acid.信函：聚脱氧鸟苷酸与聚脱氧胞苷酸的结构
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The pairing activity of stable nucleoprotein filaments made from recA protein, single-stranded DNA, and adenosine 5'-(gamma-thio)triphosphate.由recA蛋白、单链DNA和腺苷5'-（γ-硫代）三磷酸制成的稳定核蛋白细丝的配对活性。
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Topography of the interaction of recA protein with single-stranded deoxyoligonucleotides.RecA蛋白与单链脱氧寡核苷酸相互作用的拓扑结构。
J Biol Chem. 1986 May 25;261(15):6954-60.
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Rapid plasmid library screening using RecA-coated biotinylated probes.使用RecA包被的生物素化探针进行快速质粒文库筛选。
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Intramolecular DNA triplexes in supercoiled plasmids.超螺旋质粒中的分子内DNA三链体
Proc Natl Acad Sci U S A. 1988 Sep;85(17):6292-6. doi: 10.1073/pnas.85.17.6292.
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Site-specific oligonucleotide binding represses transcription of the human c-myc gene in vitro.位点特异性寡核苷酸结合在体外可抑制人c-myc基因的转录。
Science. 1988 Jul 22;241(4864):456-9. doi: 10.1126/science.3293213.
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Use of psoralen-modified oligonucleotides to trap three-stranded RecA-DNA complexes and repair of these cross-linked complexes by ABC excinuclease.使用补骨脂素修饰的寡核苷酸捕获三链RecA-DNA复合物并通过ABC核酸外切酶修复这些交联复合物。
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10
Triple-strand formation in the homopurine:homopyrimidine DNA oligonucleotides d(G-A)4 and d(T-C)4.同型嘌呤：同型嘧啶DNA寡核苷酸d(G-A)4和d(T-C)4中的三链形成。
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