Baker A, Kanofsky J R
Research Service, Edward Hines Jr. Department of Veterans Affairs Hospital, Hines, IL 60141.
Photochem Photobiol. 1992 Apr;55(4):523-8. doi: 10.1111/j.1751-1097.1992.tb04273.x.
Singlet oxygen lifetimes for detergent-dispersed L1210 leukemia cells in deuterium oxide buffer were measured by following the decay of 1270 nm phosphorescence. Four photosensitizers and two detergents were studied. Stern-Volmer plots were linear over the cell concentration range studied (0-10(7) cells/mL). The singlet-oxygen quenching constants obtained depended somewhat upon the specific combination of detergent and photosensitizer used. Extrapolation of the singlet-oxygen lifetime data to "100%" cell concentration (1.39 +/- 0.04 x 10(9) cells/mL) and correction for the contribution of the water solvent gave a singlet-oxygen lifetime between 0.17 and 0.32 microseconds for the L1210 leukemia cell. The theoretical contributions of various types of biological molecules within the L1210 cell to the total singlet-oxygen quenching were calculated from their concentrations and their quenching constants. These calculations suggest that proteins will quench most of the singlet-oxygen. Only about 7% of the singlet-oxygen is quenched by water.
通过跟踪1270纳米磷光的衰减，测量了氘代氧化缓冲液中去污剂分散的L1210白血病细胞的单线态氧寿命。研究了四种光敏剂和两种去污剂。在研究的细胞浓度范围（0 - 10⁷个细胞/毫升）内，斯特恩-沃尔默图呈线性。所获得的单线态氧猝灭常数在一定程度上取决于所用去污剂和光敏剂的具体组合。将单线态氧寿命数据外推至“100%”细胞浓度（1.39 ± 0.04 × 10⁹个细胞/毫升），并校正水溶剂的贡献后，L1210白血病细胞的单线态氧寿命在0.17至0.32微秒之间。根据L1210细胞内各种生物分子的浓度及其猝灭常数，计算了它们对总单线态氧猝灭的理论贡献。这些计算表明，蛋白质将猝灭大部分单线态氧。只有约7%的单线态氧被水猝灭。
