Nadin-Davis S A, Muldoon F, Wandeler A I
Rabies Centre of Expertise, Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency, Ottawa, Ontario, Canada.
Epidemiol Infect. 2006 Jun;134(3):534-47. doi: 10.1017/S0950268805005108. Epub 2005 Oct 5.
Three physically separate incursions of the raccoon strain of rabies have entered Canada, two into eastern Ontario in 1999 and one into New Brunswick in 2000. The course of these epizootics is described. Phylogenetic analysis of the index cases from these two provinces with raccoon rabies viruses representative of this strain in the United States supported the independence of these incursions into Canada via cross-border transmission from the United States. Genetic characterization of 190 isolates from these two Canadian provinces over a 550-bp region of the variable central portion of the viral P gene distinguished 14 variants in Ontario and five in New Brunswick although in both regions the variant represented by the initial case was most commonly encountered. The quasi-species nature of the Ontario virus was analysed using isolates taken at different times during the main outbreak to examine whether viral variation was increasing with time as well as changing in nature. These data provide a framework for study of future incursions of this rabies strain into Canada.
狂犬病浣熊毒株三次分别从物理上不同的区域侵入加拿大，两次于1999年侵入安大略省东部，一次于2000年侵入新不伦瑞克省。本文描述了这些动物疫病的发展过程。对来自这两个省份的首例病例与美国代表该毒株的浣熊狂犬病病毒进行系统发育分析，支持了这些通过从美国跨境传播而侵入加拿大的事件的独立性。对来自加拿大这两个省份的190个分离株在病毒P基因可变中心部分的550个碱基对区域进行基因特征分析，在安大略省区分出14个变体，在新不伦瑞克省区分出5个变体，尽管在这两个地区，首例病例所代表的变体最为常见。利用在主要疫情期间不同时间采集的分离株分析了安大略病毒的准种性质，以研究病毒变异是否随时间增加以及性质是否改变。这些数据为研究该狂犬病毒株未来侵入加拿大提供了一个框架。
