Lesley Scott A, Wilson Ian A
Joint Center for Structural Genomics, Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, 92121 CA, USA.
J Struct Funct Genomics. 2005;6(2-3):71-9. doi: 10.1007/s10969-005-2897-2.
By definition, structural genomics centers must be able to address a large number of diverse protein targets. The methods developed should permit parallel and cost-effective processing while allowing for the diverse nature of proteins. Our approach to this problem is a multi-tiered effort where targets are characterized and categorized by behavior and processed in parallel by appropriate methods. The Joint Center for Structural Genomics (JCSG) has applied this tactic to create a fully integrated and scaleable structure determination pipeline. Highlights of the development of the current pipeline for protein production and crystallization are presented here.
根据定义，结构基因组学中心必须能够处理大量不同的蛋白质靶点。所开发的方法应允许并行且具有成本效益的处理，同时考虑到蛋白质的多样性。我们解决这个问题的方法是多层次的努力，即根据行为对靶点进行表征和分类，并通过适当的方法并行处理。结构基因组学联合中心（JCSG）已应用此策略创建了一个完全集成且可扩展的结构测定流程。本文介绍了当前蛋白质生产和结晶流程开发的亮点。
