Williams Tatjana, Joseph Biju, Beier Dagmar, Goebel Werner, Kuhn Michael
Lehrstuhl für Mikrobiologie, Theodor Boveri-Institut für Biowissenschaften der Universität Würzburg, Germany.
FEMS Microbiol Lett. 2005 Nov 15;252(2):287-98. doi: 10.1016/j.femsle.2005.09.011. Epub 2005 Sep 22.
An isogenic mutant of Listeria monocytogenes EGD with a deletion of the response regulator gene degU showed a lack of motility due to the absence of flagella. In the present study, we used two-dimensional gel electrophoresis, mass-spectrometry and microarray analyses to identify the listerial genes that depend on DegU for expression. We found that the two L. monocytogenes operons encoding flagella-specific genes and the monocistronically transcribed flaA gene are positively regulated by DegU at 24 degrees C, but are not expressed at 37 degrees C.
单核细胞增生李斯特菌EGD的一个同源突变体缺失了应答调节基因degU，由于缺乏鞭毛而表现出运动能力缺失。在本研究中，我们使用二维凝胶电泳、质谱分析和微阵列分析来鉴定依赖DegU进行表达的李斯特菌基因。我们发现，编码鞭毛特异性基因的两个单核细胞增生李斯特菌操纵子以及单顺反子转录的flaA基因在24℃时受DegU正调控，但在37℃时不表达。
