Nicolini Claudio, Pechkova Eugenia
Fondazione EL.B.A., via delle Testuggini snc, 00187 Rome, Italy.
J Cell Biochem. 2006 Feb 15;97(3):544-52. doi: 10.1002/jcb.20537.
Ultrasmall P450scc cytochrome microcrystals are grown by classical hanging vapor diffusion and by its modification using homologous protein thin-film template displaying a long-range order. The nucleation and growth mechanisms of P450scc microcrystals are studied at the thin cytochrome film surface by a new microbeam grazing incidence small angle X-ray scattering (microGISAXS) technique developed at the microfocus beamline of the European Synchrotron Radiation Facility (ESRF) in Grenoble, France. P450scc cytochrome crystals of about 5 microm are also investigated by synchrotron radiation diffraction in order to attempt a preliminary analysis of the atomic structure of this unique protein system yet unsolved.
超小细胞色素P450scc微晶通过经典的悬滴气相扩散法及其使用具有长程有序性的同源蛋白质薄膜模板进行的改进方法生长。利用在法国格勒诺布尔欧洲同步辐射装置（ESRF）的微聚焦光束线开发的一种新型微束掠入射小角X射线散射（microGISAXS）技术，在细胞色素薄膜表面研究了P450scc微晶的成核和生长机制。还通过同步辐射衍射对约5微米的P450scc细胞色素晶体进行了研究，以便尝试对这个尚未解决的独特蛋白质系统的原子结构进行初步分析。
