Berovic Marin
Faculty of Chemistry and Chemical Engineering, Department of Chemical, Biochemical and Environmental Engineering, University of Ljubljana, Askerceva 5, 1000 Ljubljana, Slovenia.
Biotechnol Annu Rev. 2005;11:257-79. doi: 10.1016/S1387-2656(05)11008-4.
Sterilisation of fermentation media, inlet and outlet gases and maintenance of aseptic conditions during bioprocessing represents one of the most relevant parts of comprehensive upstream processing. The present chapter reviews various sterilisation techniques and methods for sterilisation of inlet gases, liquids, including sterilisation of small and large industrial equipment and validation of sterilisation procedures. Various methods for sterilisation using different kinds of filters, filter cartridges and membrane filters are also presented. Liquid sterilisation methods by filtration as well as heat sterilisation are discussed. Sterilisation of small and industrial scale equipment by chemical agents and physical methods as ionising radiation and dry heat sterilisation including the validation of sterilisation are discussed.
发酵培养基、进出气的灭菌以及生物加工过程中无菌条件的维持是全面上游加工中最关键的部分之一。本章综述了各种灭菌技术和方法，包括进气、液体的灭菌，其中涵盖了小型和大型工业设备的灭菌以及灭菌程序的验证。还介绍了使用不同类型过滤器、滤筒和膜过滤器的各种灭菌方法。讨论了通过过滤以及热灭菌进行液体灭菌的方法。探讨了使用化学试剂和物理方法（如电离辐射和干热灭菌）对小型和工业规模设备进行灭菌，包括灭菌验证。
