Xing Jianhua, Liao Jung-Chi, Oster George
Departments of Molecular Cell Biology, University of California, Berkeley, CA 94720-1132, USA.
Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16539-46. doi: 10.1073/pnas.0507207102. Epub 2005 Oct 10.
We present a mesoscopic model for ATP synthesis by F(1)F(o) ATPase. The model combines the existing experimental knowledge of the F(1) enzyme into a consistent mathematical model that illuminates how the stages in synthesis are related to the protein structure. For example, the model illuminates how specific interactions between the gamma, epsilon, and alpha(3)beta(3) subunits couple the F(o) motor to events at the catalytic sites. The model also elucidates the origin of ADP inhibition of F(1) in its hydrolysis mode. The methodology we develop for constructing the structure-based model should prove useful in modeling other protein motors.
我们提出了一种用于F(1)F(o)ATP合酶合成ATP的介观模型。该模型将F(1)酶现有的实验知识整合到一个一致的数学模型中,阐明了合成过程中的各个阶段与蛋白质结构之间的关系。例如,该模型阐明了γ、ε和α(3)β(3)亚基之间的特定相互作用如何将F(o)马达与催化位点的事件耦合起来。该模型还阐明了在水解模式下ADP对F(1)抑制作用的起源。我们开发的用于构建基于结构的模型的方法,在对其他蛋白质马达进行建模时应该会很有用。