Wang Q C, Hosoya K, Svec F, Fréchet J M
Department of Chemistry, Baker Laboratory, Cornell University, Ithaca, New York 14853-1301.
Anal Chem. 1992 Jun 1;64(11):1232-8. doi: 10.1021/ac00035a008.
A novel approach to monosized macroporous polymeric separation media with vastly enhanced pore size distributions and chromatographic properties has been developed. Key to this approach is the combined use of monodispersed polymeric particles and suitable solvents as porogens in the copolymerization of styrene and divinylbenzene. Following polymerization, the polymeric porogen is dissolved, leaving behind the monosized beads with a controlled pore structure. The exact pore size and pore size distribution of the final beads are largely controlled by the amount of soluble polymer in the polymerizing mixture: the larger the proportion of soluble polymer in the system, the larger the pores. The uniformly sized macroporous beads prepared with an optimized ratio of polymeric and low molecular weight porogens proved to be very efficient even in short columns for the separation of polystyrene standards in the SEC mode and the separation of proteins in the reversed-phase mode. The relationship between pore size and specific surface area, on one hand, and chromatographic properties of the stationary phase, on the other, have been clearly documented.
一种制备单分散大孔聚合物分离介质的新方法已经开发出来，该介质具有大大改善的孔径分布和色谱性能。这种方法的关键在于在苯乙烯和二乙烯基苯的共聚过程中，将单分散聚合物颗粒与合适的溶剂作为致孔剂联合使用。聚合反应结束后，聚合物致孔剂被溶解，留下具有可控孔结构的单分散珠粒。最终珠粒的确切孔径和孔径分布在很大程度上由聚合混合物中可溶性聚合物的量控制：体系中可溶性聚合物的比例越大，孔径越大。用聚合物和低分子量致孔剂的优化比例制备的尺寸均匀的大孔珠粒，即使在短柱中，在尺寸排阻色谱（SEC）模式下分离聚苯乙烯标准品以及在反相模式下分离蛋白质时，也证明是非常有效的。一方面，孔径与比表面积之间的关系，另一方面，固定相的色谱性能之间的关系已经得到了明确的记录。
