拜莱青霉产柠檬酸和草酸及磷酸钙的溶解作用
Production of citric and oxalic acids and solubilization of calcium phosphate by Penicillium bilaii.
作者信息
Cunningham J E, Kuiack C
机构信息
Philom Bios, Inc., Saskatoon, Saskatchewan, Canada.
出版信息
Appl Environ Microbiol. 1992 May;58(5):1451-8. doi: 10.1128/aem.58.5.1451-1458.1992.
An isolate of Penicillium bilaii previously reported to solubilize mineral phosphates and enhance plant uptake of phosphate was studied. Using agar media with calcium phosphate and the pH indicator alizarin red S, the influence of the medium composition on phosphate solubility and medium acidification was recorded. The major acidic metabolites produced by P. bilaii in a sucrose nitrate liquid medium were found to be oxalic acid and citric acid. Citric acid production was promoted under nitrogen-limited conditions, while oxalic acid production was promoted under carbon-limited conditions. Citric acid was produced in both growth and stationary phases, but oxalic acid production occurred only in stationary phase. When submerged cultures which normally produce acid were induced to sporulate, the culture medium shifted toward alkaline rather than acid reaction with growth.
对先前报道的一种解磷青霉（Penicillium bilaii）分离株进行了研究，该菌株可溶解矿物磷酸盐并增强植物对磷酸盐的吸收。使用含有磷酸钙和pH指示剂茜素红S的琼脂培养基，记录了培养基成分对磷酸盐溶解度和培养基酸化的影响。发现解磷青霉在蔗糖硝酸盐液体培养基中产生的主要酸性代谢产物是草酸和柠檬酸。在氮限制条件下促进柠檬酸的产生，而在碳限制条件下促进草酸的产生。柠檬酸在生长阶段和稳定期均产生，但草酸仅在稳定期产生。当正常产酸的深层培养物被诱导产孢时，培养基随着生长转向碱性而非酸性反应。
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