Ager J W, Nalla R K, Breeden K L, Ritchie R O
Lawrence Berkeley National Laboratory, Materials Sciences Division, Berkeley, California 94720.
J Biomed Opt. 2005 May-Jun;10(3):034012. doi: 10.1117/1.1924668.
The age-related deterioration in bone quality and consequent increase in fracture incidence is an obvious health concern that is becoming increasingly significant as the population ages. Raman spectroscopy with deep-ultraviolet excitation (244 nm) is used to measure vibrational spectra from human cortical bone obtained from donors over a wide age range (34-99 years). The UV Raman technique avoids the fluorescence background usually found with visible and near-infrared excitation and, due to resonance Raman effects, is particularly sensitive to the organic component of bone. Spectral changes in the amide I band at 1640 cm(-1) are found to correlate with both donor age and with previously reported fracture toughness data obtained from the same specimens. These results are discussed in the context of possible changes in collagen cross-linking chemistry as a function of age, and are deemed important to further our understanding of the changes in the organic component of the bone matrix with aging.
随着人口老龄化，与年龄相关的骨质恶化以及随之而来的骨折发生率增加是一个明显的健康问题，且日益严重。采用深紫外激发（244纳米）的拉曼光谱法用于测量从广泛年龄范围（34 - 99岁）的供体获取的人类皮质骨的振动光谱。紫外拉曼技术避免了通常在可见光和近红外激发下出现的荧光背景，并且由于共振拉曼效应，对骨的有机成分特别敏感。发现在1640厘米-1处酰胺I带的光谱变化与供体年龄以及从相同标本获得的先前报道的断裂韧性数据均相关。这些结果在胶原交联化学随年龄变化的可能情况的背景下进行了讨论，并被认为对于进一步理解骨基质有机成分随衰老的变化具有重要意义。
