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发根农杆菌A4诱导黄瓜产生新型根系表型的分子基础

Molecular basis for novel root phenotypes induced by Agrobacterium rhizogenes A4 on cucumber.

作者信息

Amselem J, Tepfer M

机构信息

Laboratoire de Biologie Cellulaire, INRA-Centre de Versailles, France.

出版信息

Plant Mol Biol. 1992 Jun;19(3):421-32. doi: 10.1007/BF00023390.

DOI:10.1007/BF00023390
PMID:1623191
Abstract

We have used the wild-type Agrobacterium rhizogenes strain A4 to induce roots on cucumber stem explants. Cultures of transformed roots obtained that were capable of hormone-autonomous growth could be grouped in three phenotypic classes. Of particular interest were extremely thick roots of a type not previously described. Characterization of the transferred DNA and of the expression of the corresponding genes allowed us to determine that the genes rolABC of the TL region of the Ri plasmid are sufficient to induce thin roots similar to those observed in other species, while the aux genes of the TR region are sufficient to induce thick roots. Among clones bearing the aux genes, there was a correlation between level of expression of aux2 and root phenotype.

摘要

我们已使用野生型发根农杆菌菌株A4在黄瓜茎外植体上诱导生根。所获得的能够自主生长激素的转化根培养物可分为三个表型类别。特别令人感兴趣的是一种以前未描述过的极粗的根。对转移DNA和相应基因表达的表征使我们能够确定，Ri质粒TL区域的rolABC基因足以诱导出与其他物种中观察到的相似的细根，而TR区域的aux基因足以诱导出粗根。在携带aux基因的克隆中，aux2的表达水平与根表型之间存在相关性。

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本文引用的文献

1
Identification of T-DNA in the root-inducing plasmid of the agropine type Agrobacterium rhizogenes 1855.鉴定发根农杆菌 1855 型根诱导质粒中的 T-DNA。
Plant Mol Biol. 1982 Dec;1(4):291-300. doi: 10.1007/BF00027560.
2
Developmental regulation of RI TL-DNA gene expression in roots, shoots and tubers of transformed potato (Solanum tuberosum cv. Desiree).转化马铃薯（Solanum tuberosum cv. Desiree）的根、茎和块茎中 RI TL-DNA 基因表达的发育调控。
Plant Mol Biol. 1986 Sep;6(5):321-30. doi: 10.1007/BF00034939.
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A negative selection scheme for tobacco protoplast-derived cells expressing the T-DNA gene 2.
发根农杆菌A4介导的娃儿藤遗传转化：不同转化根克隆的生长及娃儿藤碱产量
Plant Cell Rep. 2005 Apr;24(1):25-35. doi: 10.1007/s00299-004-0904-x. Epub 2005 Jan 20.
4
Multiple regions of a divergent promoter control the expression of the Agrobacterium rhizogenes aux1 and aux2 plant oncogenes.一个分歧启动子的多个区域控制发根农杆菌aux1和aux2植物致癌基因的表达。
Mol Gen Genet. 1993 May;239(1-2):225-34. doi: 10.1007/BF00281622.
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Expression of Agrobacterium rhizogenes auxin biosynthesis genes in transgenic tobacco plants.发根农杆菌生长素生物合成基因在转基因烟草植株中的表达。
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一种用于表达 T-DNA 基因 2 的烟草原生质体细胞的负选择方案。
Plant Cell Rep. 1988 Jan;7(1):63-6. doi: 10.1007/BF00272980.
4
Roots induced on cucumber cotyledons by the agropine Ri plasmid TR-DNA exhibit the transformed phenotype.由农杆菌 Ri 质粒 TR-DNA 诱导的黄瓜子叶生根表现出转化表型。
Plant Cell Rep. 1991 Mar;9(11):647-50. doi: 10.1007/BF00231808.
5
Agrobacterium rhizogenes T-DNA genes capable of inducing hairy root phenotype.能够诱导毛状根表型的发根农杆菌T-DNA基因。
Mol Gen Genet. 1987 Oct;209(3):475-80. doi: 10.1007/BF00331152.
6
Independent and synergistic activity of rol A, B and C loci in stimulating abnormal growth in plants.rolA、B 和 C 基因座在刺激植物异常生长中的独立和协同活性。
EMBO J. 1987 Dec 20;6(13):3891-9. doi: 10.1002/j.1460-2075.1987.tb02729.x.
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Ribonucleic acid isolated by cesium chloride centrifugation.通过氯化铯离心法分离得到的核糖核酸。
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