聚（丁二酸丁二醇酯-共-己二酸丁二醇酯）降解微生物的分离与鉴定

Isolation and characterization of poly(butylene succinate-co-butylene adipate)-degrading microorganism.

作者信息

Hayase Nobuki, Yano Hidetoshi, Kudoh Emi, Tsutsumi Chikara, Ushio Kazutoshi, Miyahara Yasushi, Tanaka Suminori, Nakagawa Katsuhiko

机构信息

Department of Applied Chemistry and Biotechnology, Niihama National College of Technology, Niihama, Ehime 792-8580, Japan.

出版信息

J Biosci Bioeng. 2004;97(2):131-3. doi: 10.1016/S1389-1723(04)70180-2.

DOI:10.1016/S1389-1723(04)70180-2

PMID:16233604

Abstract

Poly(butylene succinate-co-butylene adipate) (PBSA)-degrading bacterium, strain 1-A, was isolated from soil. Strain 1-A was identified as Bacillus pumilus on the basis of its physiological properties and partial 16S rRNA gene sequence. Strain 1-A also degraded poly(butylene succinate) (PBS) and poly(epsilon-caprolactone). On the other hand, poly(butylene adipate terephthalate) and poly(lactic acid) were minimally degraded by strain 1-A. The NMR spectra of degradation products from PBSA indicated that the adipate units were more rapidly degraded than 1,4-butanediol and succinate units. This seems to be one of the reasons why strain 1-A degraded PBSA faster than PBS.

摘要

从土壤中分离出了一株聚（丁二酸丁二醇酯 - 共 - 己二酸丁二醇酯）（PBSA）降解菌，菌株1 - A。根据其生理特性和部分16S rRNA基因序列，菌株1 - A被鉴定为短小芽孢杆菌。菌株1 - A还能降解聚（丁二酸丁二醇酯）（PBS）和聚（ε - 己内酯）。另一方面，聚（对苯二甲酸丁二醇酯）和聚（乳酸）被菌株1 - A的降解程度极小。PBSA降解产物的核磁共振谱表明，己二酸酯单元比1,4 - 丁二醇和琥珀酸酯单元降解得更快。这似乎是菌株1 - A降解PBSA比PBS更快的原因之一。