Li B, Yang P, Zhou H, Huang X, Jin H, Chu L, Gao Y, Zhu L, Kijlstra A
Zhongshan Ophthalmic Center, Sun Yat-sen University, 54 Xianlie Road, Guangzhou 510060, P R China.
Br J Ophthalmol. 2005 Nov;89(11):1410-2. doi: 10.1136/bjo.2005.074062.
To test the hypothesis that T-bet expression is altered in patients with Vogt-Koyanagi-Harada (VKH) disease.
Peripheral blood was withdrawn from 16 VKH patients before and after immunosuppressive treatment and from 16 healthy individuals. IFN-gamma, IL-2, and IL-4 in the serum and the supernatants of peripheral blood mononuclear cells (PBMC) cultured with or without phytohaemagglutinin (PHA) were measured by ELISA. T-bet mRNA and protein expression in PBMC cultured with or without PHA was detected by RT-PCR and western blot, respectively.
The level of IFN-gamma, but not IL-2 and IL-4, was significantly higher in the supernatants of stimulated PBMC in patients than in controls. A significantly increased T-bet mRNA was found in VKH patients during an active uveitis episode, but not in quiescent patients, compared to controls. T-bet protein was detectable in VKH patients during an active uveitis episode, but not in quiescent patients nor in the healthy controls. Stimulation of PBMC with PHA resulted in a marked upregulation of T-bet mRNA and protein expression for both patients and controls with no significant difference between the two groups.
Upregulation of T-bet may be associated with the development of a Th1 mediated immune response in VKH disease.
验证Vogt-小柳原田（VKH）病患者中T-bet表达发生改变这一假说。
采集16例VKH病患者免疫抑制治疗前后的外周血以及16名健康个体的外周血。采用酶联免疫吸附测定法（ELISA）检测血清以及经或未经植物血凝素（PHA）培养的外周血单个核细胞（PBMC）上清液中的γ干扰素、白细胞介素-2和白细胞介素-4。分别采用逆转录-聚合酶链反应（RT-PCR）和蛋白质免疫印迹法检测经或未经PHA培养的PBMC中T-bet mRNA和蛋白表达。
患者受刺激PBMC上清液中γ干扰素水平显著高于对照组，而白细胞介素-2和白细胞介素-4水平无差异。与对照组相比，VKH病患者在葡萄膜炎活动期T-bet mRNA显著增加，而静止期患者则无此现象。VKH病患者在葡萄膜炎活动期可检测到T-bet蛋白，而静止期患者和健康对照组均未检测到。PHA刺激PBMC后，患者和对照组的T-bet mRNA和蛋白表达均显著上调，两组间无显著差异。
T-bet上调可能与VKH病中Th1介导免疫反应的发生有关。
