Li Bin, Abliz Zeper, Tang Meijun, Fu Guangmiao, Yu Shishan
The Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
J Chromatogr A. 2006 Jan 6;1101(1-2):53-62. doi: 10.1016/j.chroma.2005.09.058. Epub 2005 Oct 19.
Triterpenoid saponins in bioactive crude extract from Symplocos chinensis were rapidly identified using electrospray ionization multi-stage tandem mass spectrometry (ESI-MSn) and liquid chromatography coupled with sequential mass spectrometry (LC-MSn). According to the characteristic fragmentation behavior of known glucuronide-type triterpenoid saponins isolated from this plant, a total of fourteen constituents in the crude extract were structurally characterized on the basis of their retention time and tandem mass spectrometric analysis, including five pairs of naturally occurring isomers. Except one known saponin formerly obtained, the other constituents were new compounds. The analytical method of LC-MSn combined with ESI-MSn in positive and negative ion modes has been developed for the direct structural elucidation of triterpenoid saponins of this kind in plant extracts.
采用电喷雾电离多级串联质谱（ESI-MSn）和液相色谱-串联质谱联用（LC-MSn）技术，对华山矾生物活性粗提物中的三萜皂苷进行了快速鉴定。根据从该植物中分离得到的已知葡萄糖醛酸型三萜皂苷的特征裂解行为，基于保留时间和串联质谱分析，对粗提物中的14种成分进行了结构表征，其中包括5对天然异构体。除了一种先前得到的已知皂苷外，其他成分均为新化合物。已建立了LC-MSn结合ESI-MSn在正、负离子模式下的分析方法，用于直接阐明植物提取物中此类三萜皂苷的结构。
