Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, No. 88 Yuquan Road, Tianjin 300193, PR China. hanlifeng
J Pharm Biomed Anal. 2011 Jul 15;55(5):996-1009. doi: 10.1016/j.jpba.2011.04.002. Epub 2011 Apr 8.
Ultra high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC/ESI-Q-TOF-MS/MS) was applied to separate and identify triterpenoid saponins in crude extract from the stem bark of Albizia julibrissin Durazz. The molecular weights were determined by comparing quasi-molecular ions M+NH(4) in positive mode and M-H and M-2H ions in negative mode. The MS/MS spectra of the M-H ions for saponins provided a wealth of structural information related to aglycone skeletons, sugar types and linked sequence. On the basis of the fragmentation behavior of known saponins isolated before, saponins from this plant were identified, even though references were not available. As a result, a total of twenty-eight saponins in the crude extract were identified, which all had a common basic skeleton of the triterpene oleanolic acid and eight of them were new compounds.
超高效液相色谱-电喷雾电离四极杆飞行时间串联质谱联用（UHPLC/ESI-Q-TOF-MS/MS）用于分离和鉴定合欢树皮粗提物中的三萜皂苷。通过比较正模式下的准分子离子[M+NH4]+和负模式下的[M-H]−和[M-2H]2−离子，确定分子量。[M-H]−离子的 MS/MS 谱为苷元骨架、糖的类型和连接顺序提供了丰富的结构信息。基于之前分离的已知皂苷的裂解行为，对该植物中的皂苷进行了鉴定，尽管没有参考文献。结果，在粗提物中鉴定出了总共 28 种皂苷，它们都具有三萜齐墩果酸的共同基本骨架，其中 8 种是新化合物。
