Ertuğrul Sevgi, Dönmez Gönül, Takaç Serpil
Ankara University, Faculty of Science, Department of Biology, 06100 Beşevler, Ankara, Turkey.
J Hazard Mater. 2007 Nov 19;149(3):720-4. doi: 10.1016/j.jhazmat.2007.04.034. Epub 2007 Apr 18.
The bacteria that could grow on media containing olive mill wastewater (OMW) were isolated and their lipase production capacities were investigated. The strain possessing the highest lipase activity among 17 strains grown on tributyrin agar medium was identified as Bacillus sp. The effect of initial pH on the lipase activity was investigated in tributyrin medium and pH 6 was found to be the optimal. The liquid medium composition was improved by replacing tributyrin with various carbon sources. Among the media containing different compositions of triolein, trimyristin, trilaurin, tricaprin, tricaprylin, tributyrin, triacetin, Tween 80, OMW, glucose, and whey; the medium contained 20% whey +1% triolein was found to give the highest lipase activity. Cultivation of Bacillus sp. in the optimal medium at pH 6 and 30 degrees C for 64h resulted in the extracellular and intracellular lipase activities of 15 and 168U/ml, respectively.
分离出能在含有橄榄油厂废水（OMW）的培养基上生长的细菌，并研究了它们的脂肪酶生产能力。在三丁酸甘油酯琼脂培养基上生长的17株菌株中，脂肪酶活性最高的菌株被鉴定为芽孢杆菌属。在三丁酸甘油酯培养基中研究了初始pH对脂肪酶活性的影响，发现pH 6为最佳值。通过用各种碳源替代三丁酸甘油酯来改进液体培养基组成。在含有不同组成的三油酸甘油酯、三肉豆蔻酸甘油酯、三月桂酸甘油酯、三癸酸甘油酯、三辛酸甘油酯、三丁酸甘油酯、三乙酸甘油酯、吐温80、OMW、葡萄糖和乳清的培养基中；发现含有20%乳清+1%三油酸甘油酯的培养基脂肪酶活性最高。在pH 6和30℃的最佳培养基中培养芽孢杆菌属64小时，细胞外和细胞内脂肪酶活性分别为15和168U/ml。
