Weichert Wilko, Kristiansen Glen, Schmidt Mathias, Gekeler Volker, Noske Aurelia, Niesporek Silvia, Dietel Manfred, Denkert Carsten
Institute of Pathology, Charite University Hospital, Schumannstrabe 20/21, Berlin 10117, Germany.
World J Gastroenterol. 2005 Sep 28;11(36):5644-50. doi: 10.3748/wjg.v11.i36.5644.
To clarify the expression patterns and prognostic implications of the mitotic regulator Polo-like kinase 1 (PLK1) in colon cancer.
Expression of PLK1 was investigated by immunohistochemistry (158 cases) and immunoblotting in tissue of colon adenomas and adenocarcinomas. PLK1 expression patterns were correlated with clinicopathological parameters and patient prognosis. In addition, expression of PLK1 was evaluated by immunoblot and PCR in colon carcinoma cell lines, and coexpression of PLK1 with the proliferation marker Ki-67 was investigated.
Weak PLK1 expression was observed in normal colon mucosa and adenomas. In contrast, 66.7% of carcinomas showed strong expression of PLK1. Overexpression of PLK1 correlated positively with Dukes stage (P<0.001), tumor stage (P = 0.001) and nodal status (P<0.05). Additionally, PLK1 expression was a prognostic marker in univariate survival analysis (P<0.01) and had independent prognostic significance (RR = 3.3, P = 0.02) in patients with locoregional disease. Expression of PLK1 mRNA and protein was detected in all cell lines investigated. Coexpression of PLK1 and Ki-67 was observed in the majority of colon cancer cells, but a considerable proportion of cells showed PLK1 positivity without Ki-67 expression.
PLK1 is a new prognostic marker for colon carcinoma patients and may be involved in tumorigenesis and progression of colon cancer. Strategies focusing on PLK1 inhibition in vivo might therefore represent a promising new therapeutic approach for this tumor entity.
阐明有丝分裂调节因子Polo样激酶1（PLK1）在结肠癌中的表达模式及其预后意义。
采用免疫组织化学法（158例）和免疫印迹法研究PLK1在结肠腺瘤和腺癌组织中的表达。将PLK1表达模式与临床病理参数及患者预后相关联。此外，通过免疫印迹和PCR评估PLK1在结肠癌细胞系中的表达，并研究PLK1与增殖标志物Ki-67的共表达情况。
在正常结肠黏膜和腺瘤中观察到PLK1表达较弱。相比之下，66.7%的癌组织显示PLK1强表达。PLK1的过表达与Dukes分期（P<0.001）、肿瘤分期（P = 0.001）和淋巴结状态（P<0.05）呈正相关。此外，在单因素生存分析中，PLK1表达是一个预后标志物（P<0.01），并且在局部病变患者中具有独立的预后意义（RR = 3.3，P = 0.02）。在所研究的所有细胞系中均检测到PLK1 mRNA和蛋白的表达。在大多数结肠癌细胞中观察到PLK1和Ki-67的共表达，但相当一部分细胞显示PLK1阳性而无Ki-67表达。
PLK1是结肠癌患者的一种新的预后标志物，可能参与结肠癌的发生和发展。因此，针对体内PLK1抑制的策略可能代表了针对这种肿瘤实体的一种有前景的新治疗方法。
