Ninawe S, Kuhad R C
Department of Biotechnology, Ministry of Science and Technology, Government of India, New Delhi, India.
J Appl Microbiol. 2005;99(5):1141-8. doi: 10.1111/j.1365-2672.2005.02711.x.
The present study aimed at optimization of cultural and nutritional parameters for enhanced production of xylanase from Streptomyces cyaneus SN32.
The xylanase production by S. cyaneus SN32 on most of the agro-residues tested in this study was more, as compared with the xylanase yield in the medium supplemented with commercial xylan. The presence of wheat bran as carbon source in the medium induced the highest production of xylanase followed by corn cob. Utilization of maize stalk, gram husk and black gram husk for microbial xylanase production has been reported first time in the present study. Among all the organic and inorganic sources of nitrogen tested in the study, peptone was found to be the best in stimulating xylanase production by S. cyaneus SN32.
The production of xylanase from this thermoalkalophilic actinomycete has been enhanced 1.44-fold. To the best of our knowledge, the magnitude of enzyme yield i.e. 720 IU ml(-1) by S. cyaneus SN32 has not been reported for any other actinomycete so far.
Present studies revealed that thermoalkalophilic S. cyaneus SN32, because of its simple nutritional requirements and its ability to exhibit considerably good enzyme yield, is a potent xylanase producer for its economical application in various industries.
本研究旨在优化培养和营养参数，以提高蓝链霉菌SN32木聚糖酶的产量。
与添加商业木聚糖的培养基中的木聚糖酶产量相比，本研究中测试的大多数农业残留物上蓝链霉菌SN32产生的木聚糖酶更多。培养基中以麦麸作为碳源诱导产生的木聚糖酶产量最高，其次是玉米芯。本研究首次报道了利用玉米秸秆、鹰嘴豆壳和黑鹰嘴豆壳生产微生物木聚糖酶。在本研究测试的所有有机和无机氮源中，蛋白胨被发现是刺激蓝链霉菌SN32产生木聚糖酶的最佳氮源。
这种嗜热嗜碱放线菌产生的木聚糖酶产量提高了1.44倍。据我们所知，迄今为止，尚未有其他放线菌报道过蓝链霉菌SN32的酶产量达到720 IU ml(-1) 。
目前的研究表明，嗜热嗜碱的蓝链霉菌SN32由于其简单的营养需求和产生相当高酶产量的能力，是一种在各行业具有经济应用价值的高效木聚糖酶生产者。
