Williams Jill A, Pearson Gavin J, Colles M John
Department of Biomaterials in Relation to Dentistry, Medical Science Building, Queen Mary University of London, Mile End Road, London E1 4NS, UK.
J Dent. 2006 Jul;34(6):363-71. doi: 10.1016/j.jdent.2005.08.002. Epub 2005 Oct 18.
To measure antibacterial action of photoactivated disinfection (PAD) on endodontic bacteria in planktonic suspension and root canals.
Four bacteria, Fusobacterium nucleatum,Peptostreptococcus micros, Prevotella intermedia and Streptococcus intermedius, were tested in suspension. After mixing equal volumes of Tolonium chloride and bacterial suspension for 60s, each 200 microL of concentration (>10(6)cfu mL(-1)) was irradiated with light at 633+/-2 nm. Each energy dose/Tolonium chloride concentration combination was tested eight times, with controls. Prepared root canals in Training Blocs and extracted human teeth were inoculated with S. intermedius followed by 10 mg L(-1) Tolonium chloride or saline. Bacteria in canals were sampled before and after light irradiation. Student t-test assessed significance of changes in viable bacteria produced by treatment of either light or Tolonium chloride alone and light/Tolonium chloride combinations.
In suspension, reductions in bacteria were highly significant (P<0.01) for light/Tolonium chloride combinations compared to light or Tolonium chloride alone. Maximum mean log reductions of 1.14 (P. intermedia), 2.48 (P. micros), 2.81 (F. nucleatum) and 6.73 (S. intermedius) were at 4.8 J/20 mg L(-1). Antibacterial action was increased by energy dose increase (not always significantly), but not by Tolonium chloride concentration. In control canals mean log reductions of 0.42 (Blocs) and 0.38 (teeth) from initial levels were not significant. PAD mean log reductions of 2.40 (Blocs) and 2.01 (teeth) were highly significant. Changes for PAD/energy dose combinations were not significant.
PAD killed endodontic bacteria at statistically significant levels compared to controls. Kills varied with bacterial species.
测定光活化消毒（PAD）对浮游菌悬液和根管内牙髓细菌的抗菌作用。
对具核梭杆菌、微小消化链球菌、中间普氏菌和中间链球菌这四种细菌进行悬液测试。将等量的氯化甲苯胺蓝和细菌悬液混合60秒后，取每200微升浓度（>10⁶ cfu/mL⁻¹）的混合液用633±2纳米的光进行照射。每个能量剂量/氯化甲苯胺蓝浓度组合进行八次测试，并设对照组。在训练模块和拔除的人牙中制备根管，接种中间链球菌，然后分别加入10 mg/L⁻¹的氯化甲苯胺蓝或生理盐水。在光照前后对根管内的细菌进行采样。采用学生t检验评估单独光照、单独使用氯化甲苯胺蓝以及光照/氯化甲苯胺蓝组合处理后活菌数量变化的显著性。
在悬液中，与单独光照或单独使用氯化甲苯胺蓝相比，光照/氯化甲苯胺蓝组合使细菌数量的减少具有高度显著性（P<0.01）。在4.8 J/20 mg/L⁻¹时，中间普氏菌的最大平均对数减少量为1.14，微小消化链球菌为2.48，具核梭杆菌为2.81，中间链球菌为6.73。抗菌作用随能量剂量增加而增强（并非总是显著），但不随氯化甲苯胺蓝浓度增加而增强。在对照根管中，从初始水平的平均对数减少量，训练模块为0.42，牙齿为0.38，均无显著性。PAD处理后的平均对数减少量，训练模块为2.40，牙齿为2.01，具有高度显著性。PAD/能量剂量组合的变化无显著性。
与对照组相比，PAD能使牙髓细菌数量在统计学上显著减少。杀菌效果因细菌种类而异。
