Rai Sujay, Wahile Atul, Mukherjee Kakali, Saha Bishnu Pada, Mukherjee Pulok K
School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, 188 Raja S C Mullick Road, Kolkata 700 032, India.
J Ethnopharmacol. 2006 Apr 6;104(3):322-7. doi: 10.1016/j.jep.2005.09.025. Epub 2005 Oct 18.
Antioxidant activity of hydro alcoholic extract of Nelumbo nucifera seeds (HANN) was studied using in vitro and in vivo models. Total phenolic content in HANN was found to be 7.61 +/- 0.04% (w/w). Characteristic HPTLC fingerprints of HANN were also made using different solvent systems. The HANN exhibited strong free radical scavenging activity as evidenced by the low IC(50) values in both DPPH (1,1-diphenyl-2-picryl hydrazyl) (6.12 +/- 0.41 microg/ml) and nitric oxide (84.86 +/- 3.56 microg/ml) methods. The values were found to be less than those of rutin, the standard used. Acute toxicity of HANN was evaluated in Swiss Albino mice, no signs of toxicity were observed up to the oral dose of 1,000 mg/kg body weight. Administration of HANN to Wistar rats at 100 and 200 mg/kg body weight for 4 days prior to carbon tetrachloride (CCl(4)) treatment caused a significant dose dependent increase (p < 0.05 to p < 0.001) in the level of superoxide dismutase (SOD) and catalase and a significant decrease (p < 0.05 to p < 0.001) in the level of thiobarbituric acid reactive substances (TBARS), when compared to CCl(4) treated control in both liver and kidney. These changes observed at 100 mg/kg body weight treatment were comparable to those observed for standard Vitamin E at 50 mg/kg treatment. Nelumbo nucifera seeds contain alkaloids, saponins, phenolics and carbohydrates. The results support significant antioxidant nature of HANN.
采用体外和体内模型研究了莲种子水醇提取物（HANN）的抗氧化活性。发现HANN中的总酚含量为7.61±0.04%（w/w）。还使用不同溶剂系统制作了HANN的特征性高效薄层色谱指纹图谱。HANN表现出较强的自由基清除活性，在二苯基苦味酰基自由基（DPPH）（1,1 - 二苯基 - 2 - 苦基肼）法（6.12±0.41微克/毫升）和一氧化氮法（84.86±3.56微克/毫升）中均具有较低的半数抑制浓度（IC50）值，这些值低于所用标准芦丁的值。在瑞士白化小鼠中评估了HANN的急性毒性，口服剂量达1000毫克/千克体重时未观察到毒性迹象。在四氯化碳（CCl4）处理前4天，以100和200毫克/千克体重的剂量给Wistar大鼠施用HANN，与CCl4处理的对照组相比，肝脏和肾脏中超氧化物歧化酶（SOD）和过氧化氢酶水平显著剂量依赖性升高（p<0.05至p<0.001），硫代巴比妥酸反应性物质（TBARS）水平显著降低（p<0.05至p<0.001）。在100毫克/千克体重处理时观察到的这些变化与在50毫克/千克处理的标准维生素E中观察到的变化相当。莲种子含有生物碱、皂苷、酚类和碳水化合物。结果支持HANN具有显著的抗氧化特性。
