Badami Shrishailappa, Moorkoth Sudheer, Rai Sujay Rammanoharsingh, Kannan Elango, Bhojraj Suresh
J. S. S. College of Pharmacy, Tamilnadu, India.
Biol Pharm Bull. 2003 Nov;26(11):1534-7. doi: 10.1248/bpb.26.1534.
Antioxidant activity of Caesalpinia sappan heartwood was studied both by in vitro and in vivo models. The ethyl acetate, methanol and water extracts exhibited strong antioxidant activity as evidenced by the low IC50 values in both 1,1-diphenyl-2-picryl hydrazyl (DPPH) and nitric oxide methods. The values were found to be less or comparable to those of ascorbic acid and rutin, the standards used. Administration of the successive methanol and water extracts at 50 and 100 mg/kg body weight given for four days prior to carbon tetrachloride (CCl4) treatment caused a significant increase in the level of superoxide dismutase (SOD) and catalase and a significant decrease in the level of thiobarbituric acid reactive substances (TBARS), when compared to CCl4 treated control in both liver and kidney. These changes observed at 100 mg/kg body weight treatment were comparable to those observed for standard vitamin E at 50 mg/kg treatment. The results support significant antioxidant nature of Caesalpinia sappan heartwood extracts.
采用体外和体内模型研究了苏木心材的抗氧化活性。乙酸乙酯、甲醇和水提取物均表现出较强的抗氧化活性，这在1,1-二苯基-2-苦基肼（DPPH）法和一氧化氮法中均表现为较低的半数抑制浓度（IC50）值。这些值低于或与所使用的标准物质抗坏血酸和芦丁的值相当。在四氯化碳（CCl4）处理前四天，以50和100mg/kg体重连续给予甲醇和水提取物，与CCl4处理的对照组相比，肝脏和肾脏中超氧化物歧化酶（SOD）和过氧化氢酶水平显著升高，硫代巴比妥酸反应性物质（TBARS）水平显著降低。在100mg/kg体重处理时观察到的这些变化与在50mg/kg处理的标准维生素E中观察到的变化相当。结果支持苏木心材提取物具有显著的抗氧化特性。
