Hanson Lynda, Boyd Amy, Johnson Margaret A T, Bennett Michael D
Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, UK.
Ann Bot. 2005 Dec;96(7):1315-20. doi: 10.1093/aob/mci283. Epub 2005 Oct 20.
A key target set at the second Plant Genome Size Workshop, held at the Royal Botanic Gardens, Kew in 2003, was to produce first DNA C-value data for an additional 1 % of angiosperm species, and, within this, to achieve 75 % familial coverage overall (up from approx. 50 %) by 2009. The present study targeted eudicot families for which representation in 2003 (42.5 %) was much lower than monocot (72.8 %) and basal angiosperm (69.0 %) families.
Flow cytometry or Feulgen microdensitometry were used to estimate nuclear DNA C-values, and chromosome counts were obtained where possible.
First nuclear DNA C-values are reported for 20 angiosperm families, including 18 eudicots. This substantially increases familial representation to 55.2 % for angiosperms and 48.5 % for eudicots.
The importance of targeting specific plant families to improve familial nuclear DNA C-value representation is reconfirmed. International collaboration will be increasingly essential to locate and obtain material of unsampled plant families, if the target set by the second Plant Genome Size Workshop is to be met.
2003年在英国皇家植物园邱园举办的第二届植物基因组大小研讨会上设定的一个关键目标是，获取另外1%被子植物物种的首批DNA C值数据，并且到2009年在这之中总体实现75%的科覆盖度（从约50%提高）。本研究针对的是2003年时代表性（42.5%）远低于单子叶植物（72.8%）和基部被子植物（69.0%）科的真双子叶植物科。
使用流式细胞术或福尔根显微密度测定法估算核DNA C值，并尽可能进行染色体计数。
报告了20个被子植物科的首批核DNA C值，包括18个真双子叶植物科。这大幅提高了被子植物的科代表性至55.2%，真双子叶植物的科代表性至48.5%。
再次证实了针对特定植物科以提高科水平核DNA C值代表性的重要性。如果要实现第二届植物基因组大小研讨会设定的目标，国际合作对于查找和获取未采样植物科的材料将变得越来越至关重要。
