Higa-Nishiyama Arisa, Takahashi-Ando Naoko, Shimizu Tsutomu, Kudo Toshiaki, Yamaguchi Isamu, Kimura Makoto
Laboratory for Remediation Research, Plant Science Center, RIKEN, 1-7-22 Suehiro, Kanagawa, Tsurumi, Yokohama 230-0045, Japan.
Transgenic Res. 2005 Oct;14(5):713-7. doi: 10.1007/s11248-005-6633-2.
Zearalenone (ZEN) is an estrogenic mycotoxin produced by the necrotrophic cereal pathogen Fusarium graminearum. This mycotoxin is detoxified by ZHD101, a lactonohydrolase from Clonostachys rosea, or EGFP:ZHD101, its fusion to the C-terminus of an enhanced green fluorescence protein. We previously showed that egfp:zhd101 is efficiently expressed in T(0) leaves of rice. In this study, we assessed the feasibility of in planta detoxification of the mycotoxin using progeny. When protein extract from T(1) leaves was incubated with ZEN, the amount of the toxin decreased significantly as measured by HPLC. ZEN degradation activity was also detected in vivo in transgenic T(2) seeds. These results suggest that zhd101 can be exploited as an efficient and cost-effective system for protection of important cereals that are more susceptible to the pathogen (e.g., wheat and maize) from contamination with the estrogenic mycotoxin.
玉米赤霉烯酮（ZEN）是由谷物坏死性病原菌禾谷镰刀菌产生的一种具有雌激素活性的霉菌毒素。这种霉菌毒素可被来自粉红粘帚霉的内酯水解酶ZHD101或其与增强型绿色荧光蛋白C末端融合的EGFP:ZHD101解毒。我们之前表明egfp:zhd101在水稻的T(0)叶片中高效表达。在本研究中，我们评估了利用子代在植物体内对该霉菌毒素进行解毒的可行性。当将T(1)叶片的蛋白质提取物与ZEN一起孵育时，通过高效液相色谱法测定，毒素的量显著减少。在转基因T(2)种子的体内也检测到了ZEN降解活性。这些结果表明，zhd101可被开发成一种高效且经济有效的系统，用于保护更易受该病原菌侵染的重要谷物（如小麦和玉米）免受雌激素性霉菌毒素的污染。
