幽门螺杆菌在各种运输条件下的最佳存活情况。
Optimal survival of Helicobacter pylori under various transport conditions.
作者信息
Soltesz V, Zeeberg B, Wadström T
机构信息
Department of Medical Microbiology, University of Lund, Sweden.
出版信息
J Clin Microbiol. 1992 Jun;30(6):1453-6. doi: 10.1128/jcm.30.6.1453-1456.1992.
Abstract
The ability of clinical isolates and type strains of Helicobacter pylori to survive in Stuart transport medium, isotonic saline solution, and urea-containing isotonic saline was evaluated. The influences of temperature (4, 10, 15, 20, and 30 degrees C) and holding time (6 to 48 h) and the effect of exposure to air on survival were also studied. The recovery rate of H. pylori was highest from Stuart transport medium in comparison with the recoveries from the other transport media tested. We found that at holding temperatures above 15 degrees C the organisms became noncultivable within 6 h or less, while they survived for 2 days or longer at 10 degrees C. The presence of urea at a concentration of 2% (wt/vol) in isotonic saline resulted in the loss of viability of the organisms tested.
摘要
评估了幽门螺杆菌临床分离株和标准菌株在斯图尔特转运培养基、等渗盐溶液和含尿素等渗盐溶液中的存活能力。还研究了温度（4、10、15、20和30摄氏度）、保存时间（6至48小时）以及暴露于空气中对存活的影响。与其他测试的转运培养基相比，幽门螺杆菌从斯图尔特转运培养基中的回收率最高。我们发现，在高于15摄氏度的保存温度下，这些微生物在6小时或更短时间内变得不可培养，而在10摄氏度下它们存活了2天或更长时间。等渗盐溶液中2%（重量/体积）浓度的尿素导致受试微生物丧失活力。
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Campylobacter pylori, the spiral bacterium associated with human gastritis, is not a true Campylobacter sp.幽门螺杆菌，这种与人类胃炎相关的螺旋状细菌，并非真正的弯曲菌属细菌。
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