在琼脂穿刺物中维持幽门螺杆菌培养。
Maintenance of Helicobacter pylori cultures in agar stabs.
机构信息
Department of Pediatrics, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
出版信息
Helicobacter. 2010 Oct;15(5):477-80. doi: 10.1111/j.1523-5378.2010.00769.x.
BACKGROUND
Helicobacter pylori requires frequent passage at 37 °C with reduced oxygen tension to maintain viability, and recovery from frozen stocks can be unpredictable and slow. Agar stab cultures were assessed as a possible means of maintaining viability without the need to passage every 4-7 days.
MATERIALS AND METHODS
Agar stabs prepared from either Brucella or Brain Heart Infusion media were inoculated deeply with H. pylori strains or H. felis and grown under varying conditions for up to 13 weeks. Subcultures were prepared from these stabs at various intervals to test for viability.
RESULTS
Established cultures in agar stabs failed to survive at room temperature but did survive at 37 °C with 10% CO₂ for up to 56 days. H. felis remained viable for up to 28 days. No difference was observed between the two media formulations.
CONCLUSION
H. pylori grown in agar stabs remains viable for prolonged periods of time without the need to subculture and may represent an improved method for storing H. pylori for infrequent use.
背景
幽门螺杆菌需要在 37°C 且低氧张力下频繁传代以保持活力，从冷冻库存中恢复可能不可预测且缓慢。琼脂刺培养被评估为一种无需每 4-7 天传代即可维持活力的可能方法。
材料和方法
用布鲁氏菌或脑心浸液培养基制备的琼脂刺，用幽门螺杆菌菌株或 H. felis 进行深接种，并在不同条件下培养长达 13 周。从这些刺中制备亚培养物以测试其活力。
结果
在室温下，已建立的琼脂刺培养物无法存活，但在 37°C 下 10% CO₂中可存活长达 56 天。H. felis 可存活长达 28 天。两种培养基配方之间没有观察到差异。
结论
在琼脂刺中生长的幽门螺杆菌在无需传代的情况下可长时间保持活力，可能代表了一种用于不频繁使用的幽门螺杆菌储存的改进方法。
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