从胃活检标本中分离出幽门螺杆菌并不取决于所使用的运送培养基。
Recovery of Helicobacter pylori from gastric biopsy specimens is not dependent on the transport medium used.
作者信息
Roosendaal R, Kuipers E J, Peña A S, de Graaff J
机构信息
Department of Clinical Microbiology and Infection Control, University Hospital VU, Amsterdam, The Netherlands.
出版信息
J Clin Microbiol. 1995 Oct;33(10):2798-800. doi: 10.1128/jcm.33.10.2798-2800.1995.
The survival of six clinical isolates of Helicobacter pylori at room temperature was investigated after suspension in five different media: brucella broth with 5% lysed horse blood (BBLH), phosphate-buffered saline (PBS), 20% glucose, Stuart medium, and PBS with 10% Fildes enrichment (PBS-F). Only in BBLH and PBS-F no decrease in mean bacterial numbers was observed during the 24-h study period. No H. pylori isolates could be cultured from Stuart medium after 7 h of incubation. In contrast, the recovery rates in PBS-F or Stuart medium of H. pylori isolates from gastric tissue specimens collected from 19 H. pylori-positive patients were not significantly different even after a delay of culture of up to 24 h. Our data show that the medium composition is not critical for the survival of H. pylori within gastric tissue specimens.
将6株幽门螺杆菌临床分离株分别悬浮于5种不同培养基中，研究其在室温下的存活情况，这5种培养基分别为：含5%裂解马血的布氏肉汤（BBLH）、磷酸盐缓冲盐水（PBS）、20%葡萄糖、Stuart培养基以及含10%菲尔德富集物的PBS（PBS-F）。在24小时的研究期间，仅在BBLH和PBS-F中未观察到细菌平均数减少。孵育7小时后，无法从Stuart培养基中培养出幽门螺杆菌分离株。相比之下，即使培养延迟长达24小时，从19例幽门螺杆菌阳性患者采集的胃组织标本中分离出的幽门螺杆菌在PBS-F或Stuart培养基中的回收率也没有显著差异。我们的数据表明，培养基成分对于胃组织标本中幽门螺杆菌的存活并不关键。
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