胚胎干细胞（ES细胞）与胚胎癌细胞（EC细胞）：同一硬币的两面。

Embryonic stem (ES) cells and embryonal carcinoma (EC) cells: opposite sides of the same coin.

作者信息

Andrews P W, Matin M M, Bahrami A R, Damjanov I, Gokhale P, Draper J S

机构信息

The Centre for Stem Cell Biology and Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, UK.

出版信息

Biochem Soc Trans. 2005 Dec;33(Pt 6):1526-30. doi: 10.1042/BST0331526.

DOI:10.1042/BST0331526

PMID:16246161

Abstract

Embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and the malignant counterparts of embryonic stem (ES) cells derived from the inner cell mass of blastocyst-stage embryos, whether human or mouse. On prolonged culture in vitro, human ES cells acquire karyotypic changes that are also seen in human EC cells. They also 'adapt', proliferating faster and becoming easier to maintain with time in culture. Furthermore, when cells from such an 'adapted' culture were inoculated into a SCID (severe combined immunodeficient) mouse, we obtained a teratocarcinoma containing histologically recognizable stem cells, which grew out when the tumour was explanted into culture and exhibited properties of the starting ES cells. In these features, the 'adapted' ES cells resembled malignant EC cells. The results suggest that ES cells may develop in culture in ways that mimic changes occurring in EC cells during tumour progression.

摘要

胚胎癌（EC）细胞是畸胎癌的干细胞，是源自囊胚期胚胎内细胞团的胚胎干细胞（ES）的恶性对应物，无论是人类还是小鼠的胚胎干细胞。在体外长期培养时，人类ES细胞会出现核型变化，这在人类EC细胞中也可见。它们还会“适应”，随着培养时间的推移增殖得更快且更易于维持。此外，当将来自这种“适应”培养物的细胞接种到严重联合免疫缺陷（SCID）小鼠体内时，我们获得了一种畸胎癌，其中含有组织学上可识别的干细胞，当肿瘤被移植到培养基中时这些干细胞会生长出来，并表现出起始ES细胞的特性。在这些特征方面，“适应”的ES细胞类似于恶性EC细胞。结果表明，ES细胞在培养过程中可能会以模仿肿瘤进展过程中EC细胞发生的变化的方式发展。