Yamamoto H, Ringler D J, Miller M D, Yasutomi Y, Hasunuma T, Letvin N L
Harvard Medical School, New England Regional Primate Research Center, Southborough, MA 01772.
J Immunol. 1992 Jul 15;149(2):728-34.
An infiltration of CD8+ lymphocytes in the dermis and epidermis underlies the skin rash that commonly occurs as a primary manifestation of an AIDS virus infection. These cutaneous lymphocytes were characterized in simian immunodeficiency virus of macaques (SIVmac)-infected rhesus monkeys. Skin rash-associated lymphocytes exhibited greater lysis of SIVmac-expressing target cells and a higher cloning efficiency for SIVmac-specific effector T cells than PBL. Moreover, both SIVmac envelope- and gag-specific CTL could be readily cloned from these skin rash-associated lymphocytes. In fact, the skin rash-associated CTL exhibited the same MHC restriction and epitope specificity as those CTL derived from PBL. These studies, therefore, demonstrate that the cutaneous infiltrating CD8+ lymphocytes in SIVmac-infected rhesus monkeys include SIVmac-specific CTL. Thus, whereas virus-specific CTL are likely to represent an important mechanism for controlling AIDS virus infections, they also may play a role in the pathogenesis of the skin lesions that occur after this infection.
真皮和表皮中CD8 +淋巴细胞浸润是皮疹的基础，皮疹通常是艾滋病病毒感染的主要表现。这些皮肤淋巴细胞在感染猕猴猿免疫缺陷病毒（SIVmac）的恒河猴中得到了表征。与皮疹相关的淋巴细胞对表达SIVmac的靶细胞具有更强的裂解作用，并且与外周血淋巴细胞（PBL）相比，SIVmac特异性效应T细胞的克隆效率更高。此外，SIVmac包膜特异性和gag特异性CTL都可以很容易地从这些与皮疹相关的淋巴细胞中克隆出来。事实上，与皮疹相关的CTL表现出与源自PBL的CTL相同的MHC限制和表位特异性。因此，这些研究表明，感染SIVmac的恒河猴皮肤浸润的CD8 +淋巴细胞包括SIVmac特异性CTL。因此，虽然病毒特异性CTL可能是控制艾滋病病毒感染的重要机制，但它们也可能在这种感染后发生的皮肤病变的发病机制中起作用。
