Yasutomi Y, Reimann K A, Lord C I, Miller M D, Letvin N L
Harvard Medical School, New England Regional Primate Research Center, Southborough, Massachusetts 01772-9102.
J Virol. 1993 Mar;67(3):1707-11. doi: 10.1128/JVI.67.3.1707-1711.1993.
To assess the possible role of cytotoxic T lymphocytes (CTLs) in containing the spread of human immunodeficiency virus in acutely infected individuals, the temporal evolution of the virus-specific CD8+ lymphocyte response was defined in simian immunodeficiency virus of macaques (SIVmac)-infected rhesus monkeys. A brief period of SIVmac plasma antigenemia was seen 9 to 16 days following intravenous infection with SIVmac, ending as the absolute number of CD8+ peripheral blood lymphocytes (PBLs) increased. In a prospective assessment of the ability of CD8+ lymphocytes of these monkeys to suppress SIVmac replication in autologous PBLs, inhibitory activity was detected as early as 4 days, with a more pronounced effect 12 to 16 days following infection. SIVmac Gag- and Nef-specific CD8+ effector cell activities were demonstrable in PBLs of animals by 2 weeks following virus inoculation. In fact, SIVmac-specific CTL precursors were documented in the PBLs of rhesus monkeys 4 to 6 days after SIVmac infection. These studies indicate that AIDS virus-specific CD8+ CTLs are present in PBLs within days of infection and may play an important role in containing the early spread of virus.
为评估细胞毒性T淋巴细胞（CTL）在抑制人类免疫缺陷病毒在急性感染个体中传播方面的潜在作用，在感染猕猴猿免疫缺陷病毒（SIVmac）的恒河猴中确定了病毒特异性CD8 +淋巴细胞反应的时间演变。静脉注射SIVmac后9至16天出现短暂的SIVmac血浆抗原血症期，随着CD8 +外周血淋巴细胞（PBL）绝对数量的增加而结束。在对这些猴子的CD8 +淋巴细胞抑制自体PBL中SIVmac复制能力的前瞻性评估中，早在4天就检测到抑制活性，感染后12至16天效果更明显。接种病毒后2周，在动物的PBL中可证明SIVmac Gag和Nef特异性CD8 +效应细胞活性。事实上，在SIVmac感染后4至6天，在恒河猴的PBL中记录到SIVmac特异性CTL前体。这些研究表明，艾滋病病毒特异性CD8 + CTL在感染后数天内存在于PBL中，并可能在抑制病毒的早期传播中发挥重要作用。
