Sengers Bram G, Heywood Hannah K, Lee David A, Oomens Cees W J, Bader Dan L
Eindhoven University of Technology, Department of Biomedical Engineering, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.
J Biomech Eng. 2005 Oct;127(5):758-66. doi: 10.1115/1.1993664.
A combined experimental-numerical approach was adopted to characterize glucose and oxygen uptake and lactate production by bovine articular chondrocytes in a model system. For a wide range of cell concentrations, cells in agarose were supplemented with either low or high glucose medium. During an initial culture phase of 48 h, oxygen was monitored noninvasively using a biosensor system. Glucose and lactate were determined by medium sampling. In order to quantify glucose and oxygen uptake, a finite element approach was adopted to describe diffusion and uptake in the experimental model. Numerical predictions of lactate, based on simple relations for cell metabolism, were found to agree well for low glucose, but not for high glucose medium. Oxygen did not play a role in either case. Given the close association between chondrocyte energy metabolism and matrix synthesis, a quantifiable prediction of utilization can present a valuable contribution in the optimization of tissue engineering conditions.
采用实验与数值相结合的方法，在模型系统中表征牛关节软骨细胞对葡萄糖和氧气的摄取以及乳酸的产生。对于广泛的细胞浓度范围，琼脂糖中的细胞分别补充低葡萄糖培养基或高葡萄糖培养基。在最初的48小时培养阶段，使用生物传感器系统对氧气进行无创监测。通过培养基采样测定葡萄糖和乳酸。为了量化葡萄糖和氧气的摄取，采用有限元方法来描述实验模型中的扩散和摄取。基于细胞代谢的简单关系对乳酸进行的数值预测发现，对于低葡萄糖培养基情况吻合良好，但对于高葡萄糖培养基则不然。在这两种情况下氧气均未起作用。鉴于软骨细胞能量代谢与基质合成之间的密切关联，对利用情况进行可量化预测可为组织工程条件的优化提供有价值的贡献。
