鸭肠炎病毒DNA定量实时聚合酶链反应的开发

Development of quantitative real-time polymerase chain reaction for duck enteritis virus DNA.

作者信息

Yang Fa-Long, Jia Wen-Xiang, Yue Hua, Luo Wei, Chen Xi, Xie Yi, Zen Wei, Yang Wei-Qin

机构信息

Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, PR China.

出版信息

Avian Dis. 2005 Sep;49(3):397-400. doi: 10.1637/7338-020305R.1.

DOI:10.1637/7338-020305R.1

PMID:16252495

Abstract

Duck enteritis virus (DEV) is a herpesvirus that causes an acute, contagious, and fatal disease. In the present article, we introduce a quantitative real-time polymerase chain reaction (PCR) assay for DEV DNA using TaqMan technology and a two-step protocol. It was confirmed to be rapid, sensitive, and specific for DEV detection. The primers and probe were designed and directed to the DNA polymerase gene of DEV. The method will provide a valuable tool for rapid laboratory diagnosis of DEV infection. By virtue of its high-throughput format and its ability to accurately quantify the viral DNA, the method may be useful for large epidemiological surveys and clarification of pathogenesis, such as latency and reactivation of the virus.

摘要

鸭肠炎病毒（DEV）是一种引起急性、传染性和致命性疾病的疱疹病毒。在本文中，我们介绍了一种使用TaqMan技术和两步法方案对DEV DNA进行定量实时聚合酶链反应（PCR）检测的方法。经证实，该方法对于DEV检测具有快速、灵敏和特异的特点。引物和探针是针对DEV的DNA聚合酶基因设计的。该方法将为DEV感染的快速实验室诊断提供一个有价值的工具。凭借其高通量形式以及准确量化病毒DNA的能力，该方法可能有助于大规模流行病学调查以及阐明发病机制，例如病毒的潜伏和再激活。