Sdiri-Loulizi Khira, Hassine Mouna, Gharbi-Khelifi Hakima, Aouni Zaidoun, Chouchane Slaheddine, Sakly Nabil, Neji-Guédiche Mohamed, Pothier Pierre, Ambert-Balay Katia, Aouni Mahjoub
Laboratory of Infectious Diseases and Biological Agents, Faculty of Pharmacy, 5000, Monastir, Tunisia.
Virus Genes. 2011 Aug;43(1):6-12. doi: 10.1007/s11262-011-0600-1. Epub 2011 Apr 2.
This study investigated the prevalence of sapovirus infections in children with acute gastroenteritis in Monastir region, Tunisia, from January 2003 to April 2007. Sapovirus was characterized by sequence and phylogenetic analyses of the partial polymerase gene. From 788 fecal specimens tested, 6 (0.8%) were positive for sapovirus, of these, 4 (66.7%) were monoinfections. All sapovirus positive samples were detected in outpatient, contrary to norovirus which was significantly more frequent in hospitalized children than in outpatients (14.5 vs. 9.5%, P = 0.03). The mean age of children with sapovirus infections was 11 ± 5.56 months (range 6-19 months). Sapovirus isolates were detected in March and between September and December 2003. Fever, vomiting, abdominal pain, and dehydration were not observed in patients with sapovirus infections. Analysis of nucleotide and amino acid sequences revealed that all 6 Tunisian sapovirus strains clustered in the GGI/1 genotype and strains were identical in the region sequenced, sharing 90.2% nucleotide identity with the reference strain Sapporo/82/JP (U65427). This represents the first finding of sapovirus infections in North Africa and especially in Tunisia. The data indicate that, contrary to norovirus which can cause severe diarrhea and is an important etiologic agent in hospitalized cases, sapovirus causes mild gastroenteritis in Tunisian children.
本研究调查了2003年1月至2007年4月期间突尼斯莫纳斯提尔地区急性胃肠炎患儿中萨波病毒感染的流行情况。通过对部分聚合酶基因进行序列和系统发育分析来鉴定萨波病毒。在检测的788份粪便标本中，6份（0.8%）萨波病毒呈阳性，其中4份（66.7%）为单一感染。所有萨波病毒阳性样本均在门诊患者中检测到，与诺如病毒相反，诺如病毒在住院儿童中比门诊患者中更为常见（14.5%对9.5%，P = 0.03）。感染萨波病毒的儿童平均年龄为11±5.56个月（范围6 - 19个月）。2003年3月以及9月至12月期间检测到萨波病毒分离株。萨波病毒感染患者未观察到发热、呕吐、腹痛和脱水症状。核苷酸和氨基酸序列分析表明，所有6株突尼斯萨波病毒菌株均聚集在GGI/1基因型中，并且在所测序区域菌株相同，与参考菌株札幌/82/JP（U65427）的核苷酸同一性为90.2%。这是北非尤其是突尼斯首次发现萨波病毒感染。数据表明，与可导致严重腹泻且是住院病例重要病原体的诺如病毒不同，萨波病毒在突尼斯儿童中引起轻度胃肠炎。
