细胞因子的比色生物条形码扩增检测法
Colorimetric bio-barcode amplification assay for cytokines.
作者信息
Nam Jwa-Min, Wise Amber R, Groves Jay T
机构信息
Department of Chemistry, University of California, Berkeley, California 94720, USA.
出版信息
Anal Chem. 2005 Nov 1;77(21):6985-8. doi: 10.1021/ac0513764.
The bio-barcode amplification assay has become a powerful tool in detecting tens to hundreds of biological targets such as proteins and nucleic acids in the entire sample. However, current bio-barcode detection schemes still require many experimental steps including microarrayer-based immobilization of oligonucleotides on a glass chip, silver enhancement of immobilized gold nanoparticles on a chip, and light-scattering measurement. Here, we report a colorimetric bio-barcode method that minimizes the above requirements while detecting 30 aM concentrations of cytokines (approximately 3 orders of magnitude more sensitive than conventional nonenzymatic cytokine detection assays). The assay is based on porous microparticles, which enable loading of a large number of barcode DNA per particle, and gold nanoparticle-based colorimetric barcode detection method.
生物条形码扩增检测法已成为检测整个样本中数十至数百种生物靶标（如蛋白质和核酸）的强大工具。然而，目前的生物条形码检测方案仍需要许多实验步骤，包括基于微阵列仪将寡核苷酸固定在玻璃芯片上、对芯片上固定的金纳米颗粒进行银增强以及光散射测量。在此，我们报告了一种比色生物条形码方法，该方法在检测30 aM浓度的细胞因子时（比传统非酶细胞因子检测方法灵敏约3个数量级），将上述要求降至最低。该检测法基于多孔微粒，每个微粒能够加载大量条形码DNA，以及基于金纳米颗粒的比色条形码检测方法。
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