de Fátima Madeira Maria, de O Schubach Armando, Schubach Tânia M P, Pereira Sandro A, Figueiredo Fabiano B, Baptista Cibele, Leal Cristianni A, Melo Cíntia X, Confort Eliame M, Marzochi Mauro C A
Serviço de Parasitologia, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, FIOCRUZ, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil.
Vet Parasitol. 2006 Jun 15;138(3-4):366-70. doi: 10.1016/j.vetpar.2006.01.059. Epub 2006 Mar 6.
A parasitological study was conducted on 66 dogs seroreactive for Leishmania captured as a control measure of visceral leishmaniasis in the State of Rio de Janeiro, Brazil. Biological samples from different anatomical sites were collected during autopsy of the animals and cultured on biphasic medium (NNN/Schneider). The Leishmania isolates were characterized by isoenzyme electrophoresis. Leishmania was isolated from 80.3% of the animals: 12 animals with Leishmania (Viannia) braziliensis isolated exclusively from cutaneous lesions, 39 with L. (L.) chagasi isolated from different sites in the same animal, and 2 with simultaneous isolation of L. (V.) braziliensis from cutaneous lesions and L. (L.) chagasi from different sites. Isolation in culture revealed the absence of Leishmania parasites in 13 animals. The results obtained confirm the existence of mixed infections in dogs in Rio de Janeiro and indicate the need to complement the investigation of seroreactive dogs using methods for the parasitological diagnosis and identification of Leishmania species.
对巴西里约热内卢州66只利什曼原虫血清反应阳性的犬进行了寄生虫学研究，这些犬是作为内脏利什曼病的控制措施捕获的。在动物尸检期间从不同解剖部位采集生物样本，并在双相培养基（NNN/施奈德）上培养。利什曼原虫分离株通过同工酶电泳进行鉴定。从80.3%的动物中分离出利什曼原虫：12只动物仅从皮肤病变中分离出巴西利什曼原虫（维安尼亚利什曼原虫），39只动物从同一动物的不同部位分离出恰加斯利什曼原虫（利什曼原虫），2只动物同时从皮肤病变中分离出巴西利什曼原虫（维安尼亚利什曼原虫）和从不同部位分离出恰加斯利什曼原虫（利什曼原虫）。培养分离显示13只动物中没有利什曼原虫寄生虫。获得的结果证实了里约热内卢犬中存在混合感染，并表明需要使用利什曼原虫物种的寄生虫学诊断和鉴定方法来补充对血清反应阳性犬的调查。
