Ito Akira, Ino Kousuke, Hayashida Masao, Kobayashi Takeshi, Matsunuma Hiroshi, Kagami Hideaki, Ueda Minoru, Honda Hiroyuki
Department of Biotechnology, School of Engineering, Nagoya University, Nagoya, Japan.
Tissue Eng. 2005 Sep-Oct;11(9-10):1553-61. doi: 10.1089/ten.2005.11.1553.
Novel technologies for creating three-dimensional constructs with complex shapes would be highly useful in tissue engineering. In the present study, tubular structures were constructed using magnetic force. Magnetite nanoparticles in cationic liposomes were taken up by target cells. The magnetically labeled cells were seeded onto ultralow-attachment plates, and a magnet was placed under the wells. After 24 h of culture, the magnetically labeled cells formed a cell sheet. Subsequently, when a cylindrical magnet was rolled onto the cell sheet, the cell sheet was attracted to the magnet and formed a tube around it. The magnet was then removed, leaving behind a tubular structure. Two types of tissue were used to create tubular structures: urinary tissue, consisting of a monotypic urothelial cell layer; and vascular tissue, consisting of heterotypic layers of endothelial cells, smooth muscle cells, and fibroblasts. The present results suggest that this novel methodology using magnetite nanoparticles and magnetic force, which we have termed "magnetic force-based tissue engineering" (Mag-TE), is a promising approach to constructing tissue-engineered tubular structures.
用于构建具有复杂形状的三维结构的新技术在组织工程中非常有用。在本研究中，利用磁力构建管状结构。阳离子脂质体中的磁铁矿纳米颗粒被靶细胞摄取。将磁性标记的细胞接种到超低附着板上，并在孔下方放置一块磁铁。培养24小时后，磁性标记的细胞形成细胞片。随后，当将圆柱形磁铁滚到细胞片上时，细胞片被磁铁吸引并围绕其形成管子。然后移除磁铁，留下管状结构。使用两种类型的组织来创建管状结构：泌尿组织，由单一类型的尿路上皮层组成；以及血管组织，由内皮细胞、平滑肌细胞和成纤维细胞的异型层组成。目前的结果表明，这种使用磁铁矿纳米颗粒和磁力的新方法，我们称之为“基于磁力的组织工程”（Mag-TE），是构建组织工程管状结构的一种有前途的方法。
