Farina Marcelo, Franco Jeferson Luis, Ribas Camila Mafalda, Meotti Flávia Carla, Missau Fabiana Cristina, Pizzolatti Moacir G, Dafre Alcir Luiz, Santos Adair R S
Departamento de Bioquímica, Centro de Cieências Biológicas, Universidade Federal de Santa Catarina, 88040-900, Florianópolis, SC, Brazil.
J Pharm Pharmacol. 2005 Nov;57(11):1503-8. doi: 10.1211/jpp.57.11.0017.
We have examined the possible protective effects of Polygala paniculata extract against methylmercury (MeHg)-induced neurotoxicity in adult mice. MeHg was diluted in drinking water (40 mg L(-1), freely available) and the hydroalcoholic Polygala extract was diluted in a 150 mM NaCl solution and administered by gavage (100 mg kg(-1) b.w., twice a day). After a two-week treatment, MeHg exposure significantly inhibited glutathione peroxidase and increased glutathione reductase activity, while the levels of thiobarbituric acid reactive substances were increased in the cerebral cortex and cerebellum. These alterations were prevented by administration of Polygala extract, except for glutathione reductase activity, which remained elevated in the cerebral cortex. Behavioural interference in the MeHg-exposed animals was evident through a marked deficit in the motor performance in the rotarod task, which was completely recovered to control levels by Polygala extract co-administration. This study has shown, for the first time, the in-vivo protective effects of Polygala extract against MeHg-induced neurotoxicity. In addition, our findings encourage studies concerning the beneficial effects of P. paniculata on neurological conditions related to excitotoxicity and oxidative stress.
我们研究了黄花远志提取物对成年小鼠甲基汞（MeHg）诱导的神经毒性的潜在保护作用。将MeHg稀释于饮用水中（40 mg L(-1)，可自由摄取），水醇提取物的黄花远志稀释于150 mM NaCl溶液中，通过灌胃给药（100 mg kg(-1)体重，每日两次）。经过两周的治疗，MeHg暴露显著抑制了谷胱甘肽过氧化物酶并增加了谷胱甘肽还原酶活性，而大脑皮层和小脑中硫代巴比妥酸反应性物质的水平升高。除了大脑皮层中谷胱甘肽还原酶活性仍然升高外，黄花远志提取物的给药预防了这些改变。通过转棒试验中运动性能的明显缺陷，暴露于MeHg的动物的行为干扰很明显，而通过联合给予黄花远志提取物，运动性能完全恢复到对照水平。本研究首次表明了黄花远志提取物对MeHg诱导的神经毒性的体内保护作用。此外，我们的研究结果鼓励开展关于黄花远志对与兴奋性毒性和氧化应激相关的神经疾病有益作用的研究。
