Romeu-Nadal M, Morera-Pons S, Castellote A I, López-Sabater M C
Dept. Nutrició i Bromatologia, Centre de Referència en Tecnologia dels Aliments (CeRTA), Facultat de Farmàcia, Universitat de Barcelona, Spain.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Jan 2;830(1):41-6. doi: 10.1016/j.jchromb.2005.10.018. Epub 2005 Nov 2.
Vitamin C is an antioxidant that can be considered a possible biomarker of oxidative stability in human milk. A high-performance liquid chromatographic method was developed and validated for determining the total Vitamin C (ascorbic acid and dehydroascorbic acid) and ascorbic acid levels in human milk. This method was then compared with an enzymatic method (a Colorimetric technique) for quantifying ascorbic acid levels. Repeatability and reproducibility were acceptable for all methods. However, the high-performance liquid chromatography (HPLC) technique provided more satisfactory results than the enzymatic method due to this last method detected 37% less ascorbic acid and does not determine the total Vitamin C because of the enzymatic method cannot reduce the dehydroascorbic acid (DHA) to ascorbic acid. Furthermore, the HPLC method has the added advantages that it requires less reagents and material, and is simpler and less time consuming than the enzymatic method. In conclusion, the drawbacks of this enzymatic method would justify its substitution for a HPLC method.
维生素C是一种抗氧化剂，可被视为母乳氧化稳定性的潜在生物标志物。开发并验证了一种高效液相色谱法，用于测定母乳中总维生素C（抗坏血酸和脱氢抗坏血酸）以及抗坏血酸的水平。然后将该方法与一种用于定量抗坏血酸水平的酶法（比色技术）进行比较。所有方法的重复性和再现性均可接受。然而，高效液相色谱（HPLC）技术比酶法提供了更令人满意的结果，因为后一种方法检测到的抗坏血酸少37%，并且由于酶法无法将脱氢抗坏血酸（DHA）还原为抗坏血酸，所以不能测定总维生素C。此外，HPLC方法还有额外的优点，即所需试剂和材料较少，并且比酶法更简单、耗时更少。总之，这种酶法的缺点证明用HPLC方法替代它是合理的。
