Hernanz A
Servicio de Bioquimica, Hospital La Paz del Insalud, Madrid, Spain.
J Clin Chem Clin Biochem. 1988 Jul;26(7):459-61.
A method is described for the determination of ascorbic acid in serum, using paired-ion high performance liquid chromatography with UV spectrophotometric determination. The advantages of the present procedure are its short analysis time, ease of performance and sufficient reliability. With this method it is possible to determine as little as 10 pmol (2 ng) serum ascorbic acid without any risk of oxidation. Correlation between the peak response and injected ascorbic acid concentration was found to be linear from 10 to 600 pmol (2 to 110 ng). An analytical recovery of 94.5 +/- 6.0 (mean +/- SD) was found by measuring a standard amount of ascorbic acid added to a serum pool on different days. Serum ascorbic acid concentrations were higher in adults (64 +/- 10 mumol/l) than in children (57 +/- 10 mumol/l).
描述了一种使用离子对高效液相色谱法结合紫外分光光度法测定血清中抗坏血酸的方法。本方法的优点是分析时间短、操作简便且可靠性足够。用这种方法可以测定低至10皮摩尔（2纳克）的血清抗坏血酸，而没有任何氧化风险。发现峰响应与注入的抗坏血酸浓度之间在10至600皮摩尔（2至110纳克）范围内呈线性相关。通过在不同日期测量添加到血清库中的标准量抗坏血酸，发现分析回收率为94.5±6.0（平均值±标准差）。成人血清抗坏血酸浓度（64±10微摩尔/升）高于儿童（57±10微摩尔/升）。
