Herlocher M L, Bucher D, Webster R G
Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN 35101.
Virus Res. 1992 Mar;22(3):281-93. doi: 10.1016/0168-1702(92)90058-h.
Construction and comparison of phylogenetic trees, the standard approach to determining the host-specific lineage of influenza A virus genes is tedious and expensive. In this study, panels of monoclonal antibodies (Mabs) produced against the matrix proteins (M1) of A/WSN and A/PR/8/34 and the nucleoprotein (NP) of A/WSN were assessed for their value in identifying the hosts of origin of the M1 and NP genes in influenza virus isolates and in mapping the proteins' functional domains. Using ELISA against a broad spectrum of reference viruses, we found two Mabs against the NP (150/4 and 469/6) to be useful in determining host-specific lineage. Comparative sequence analysis placed five amino acids within the antigenic domains recognized by Mab 150/4 and two amino acids within the domains recognized by 469/6. One Mab against the NP (5/1) recognized a conserved epitope that is present on each of the 36 influenza A viruses tested. This epitope may be a type-specific determinant for influenza A viruses and an RNA binding site. Monoclonal antibodies to M1 did not discriminate among species, but they did contribute information to the construction of a functional map of M1. These results demonstrate that Mabs to defined protein epitopes can provide useful information on the molecular epidemiology of influenza viruses.
系统发育树的构建与比较，确定甲型流感病毒基因宿主特异性谱系的标准方法既繁琐又昂贵。在本研究中，评估了针对A/WSN和A/PR/8/34的基质蛋白（M1）以及A/WSN的核蛋白（NP）产生的单克隆抗体（Mab）面板，以确定它们在鉴定流感病毒分离株中M1和NP基因的宿主来源以及绘制蛋白质功能域方面的价值。通过针对广泛的参考病毒进行酶联免疫吸附测定（ELISA），我们发现两种针对NP的单克隆抗体（150/4和469/6）可用于确定宿主特异性谱系。比较序列分析确定了单克隆抗体150/4识别的抗原域内的五个氨基酸以及469/6识别的域内的两个氨基酸。一种针对NP的单克隆抗体（5/1）识别了所测试的36种甲型流感病毒中每一种都存在的保守表位。该表位可能是甲型流感病毒的型特异性决定簇和RNA结合位点。针对M1的单克隆抗体无法区分不同物种，但它们确实为构建M1功能图谱提供了信息。这些结果表明，针对特定蛋白质表位的单克隆抗体可为流感病毒的分子流行病学提供有用信息。
