甲型流感病毒变体A/FM/1/47-MA在血凝素中存在单个氨基酸替换，可控制毒力；在基质蛋白中也存在单个氨基酸替换，同样可控制毒力以及病毒生长。
The influenza virus variant A/FM/1/47-MA possesses single amino acid replacements in the hemagglutinin, controlling virulence, and in the matrix protein, controlling virulence as well as growth.
作者信息
Smeenk C A, Brown E G
机构信息
Department of Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ontario, Canada.
出版信息
J Virol. 1994 Jan;68(1):530-4. doi: 10.1128/JVI.68.1.530-534.1994.
Abstract
Genetic analysis of mouse-adapted influenza virus variant A/FM/1/47 (FM) MA has previously identified four genome segments, 4, 5, 7, and 8, that are statistically associated with virulence. On sequencing these genome segments, we found single amino acid replacements at amino acid 47 of the HA2 subunit of the hemagglutinin and at amino acid 139 of the matrix protein. Mutation was not detected in segments 5 and 8, obviating a role for these genes in FM-MA virulence. FM-MA replicates to higher titer than FM in MDCK cells and in mouse lung. FM X FM-MA reassortants were used to show that the M1 gene controlled replication in MDCK cells as well as in mouse lung.
摘要
对适应小鼠的甲型流感病毒变异株A/FM/1/47（FM）MA进行的遗传分析先前已鉴定出四个基因组片段，即4、5、7和8，它们在统计学上与毒力相关。对这些基因组片段进行测序时，我们在血凝素HA2亚基的第47位氨基酸和基质蛋白的第139位氨基酸处发现了单个氨基酸替换。在片段5和8中未检测到突变，排除了这些基因在FM-MA毒力中的作用。FM-MA在MDCK细胞和小鼠肺中的复制滴度高于FM。FM与FM-MA的重配体用于表明M1基因控制在MDCK细胞以及小鼠肺中的复制。
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