Kurvinen E, Aasvee K, Zordania R, Jauhiainen M, Sundvall J
Tallinn Children's Hospital, Estonia.
Scand J Clin Lab Invest. 2005;65(7):541-50. doi: 10.1080/0365510500208324.
Seventy children aged 6 years (34 boys, 36 girls) were studied for cardiovascular risk factors. Among the children 40 had also been investigated at birth. The aim of the study was to determine changes in serum lipoprotein parameters from birth up to preschool age and to assess the role of some relevant factors that might affect the process. An obvious association was found between serum apolipoprotein (apo) B levels, the apoB/apoA-I ratio and lipoprotein(a) (Lp(a)) levels at birth and at 6 years of age (r = 0.43; p<0.05, r = 0.73; p<0.0001 and r = 0.81; p<0.0001, respectively). Thirty percent of children who were in the top quartile by apoB or total cholesterol levels and 66.7% of those in this quartile by apoB/apoA-I ratio at birth remained in the top quartiles also in the follow-up study. The significantly higher apoB/apoA-I ratio in newborns and the apoB/apoA-I and apoB values in the 6-year-old children were observed in the carrier apoE4 isoform as compared to E3 homozygotes. A significant influence of apoE polymorphism on serum apoB/apoA-I ratio and apoB level in preschool children was confirmed by ANOVA one-way analysis of variance. In a multiple regression analysis from all the studied factors, the independent determinants of apoB level in preschool age were apoE phenotype, gestational age and Apgar score in the first minute of life. Thus, tracking of serum Lp(a), apoB, apoB/apoA-I ratio and total cholesterol levels from birth up to 6 years of age was demonstrated. The association between apoE polymorphism and serum lipoprotein parameters became more obvious after the first 6 years of life.
对70名6岁儿童（34名男孩，36名女孩）进行了心血管危险因素研究。其中40名儿童在出生时也接受了调查。本研究的目的是确定从出生到学龄前血清脂蛋白参数的变化，并评估一些可能影响这一过程的相关因素的作用。研究发现，出生时和6岁时血清载脂蛋白（apo）B水平、apoB/apoA-I比值和脂蛋白(a)（Lp(a)）水平之间存在明显关联（r分别为0.43；p<0.05，r为0.73；p<0.0001，r为0.81；p<0.0001）。出生时apoB或总胆固醇水平处于最高四分位数的儿童中，30%在随访研究中仍处于最高四分位数；出生时apoB/apoA-I比值处于该四分位数的儿童中，66.7%在随访研究中也仍处于最高四分位数。与E3纯合子相比，携带apoE4异构体的新生儿中apoB/apoA-I比值显著更高，6岁儿童中apoB/apoA-I和apoB值也显著更高。通过单因素方差分析（ANOVA）证实，apoE多态性对学龄前儿童血清apoB/apoA-I比值和apoB水平有显著影响。在对所有研究因素进行的多元回归分析中，学龄前apoB水平的独立决定因素是apoE表型、胎龄和出生后第一分钟的阿氏评分。因此，证明了从出生到6岁对血清Lp(a)、apoB、apoB/apoA-I比值和总胆固醇水平进行追踪。apoE多态性与血清脂蛋白参数之间的关联在生命的前6年之后变得更加明显。
