Department of Plant Genetics, Breeding and Biotechnology, West-Pomeranian University of Technology, Szczecin, Poland.
J Appl Genet. 2011 Aug;52(3):313-8. doi: 10.1007/s13353-011-0049-x. Epub 2011 May 11.
The Rfc1 gene controls restoration of male fertility in rye (Secale cereale L.) with sterility-inducing cytoplasm CMS-C. Two populations of recombinant inbred lines (RIL) were used in this study to identify DArT markers located on the 4RL chromosome, in the close vicinity of the Rfc1 gene. In the population developed from the 541×2020LM intercross, numerous markers tightly linked with the restorer gene were identified. This group contained 91 DArT markers and three SCARs additionally analyzed in the study. All these markers were mapped in the distance not exceeding 6 cM from the gene of interest. In the second mapping population (541×Ot1-3 intercross), only 9 DArT markers located closely to the Rfc1 gene were identified. Five of these DArT markers were polymorphic in both populations.
Rfc1 基因控制着不育细胞质 CMS-C 诱导的黑麦（Secale cereale L.）雄性可育性的恢复。本研究使用了两个重组自交系（RIL）群体，以鉴定位于 4RL 染色体上、靠近 Rfc1 基因的 DArT 标记。在由 541×2020LM 杂交开发的群体中，鉴定出了许多与恢复基因紧密连锁的标记。该组包含 91 个 DArT 标记和另外在研究中分析的三个 SCARs。所有这些标记都映射在距离目的基因不超过 6 cM 的范围内。在第二个作图群体（541×Ot1-3 杂交）中，仅鉴定到 9 个靠近 Rfc1 基因的 DArT 标记。其中 5 个 DArT 标记在两个群体中都是多态性的。
