Weon Hang-Yeon, Kim Byung-Yong, Kwon Soon-Wo, Park In-Cheol, Cha In-Bae, Tindall Brian J, Stackebrandt Erko, Trüper Hans G, Go Seung-Joo
Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration (RDA), Suwon 441-707, Republic of Korea.
Genetic Resources Division, Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Republic of Korea.
Int J Syst Evol Microbiol. 2005 Nov;55(Pt 6):2297-2302. doi: 10.1099/ijs.0.63741-0.
A bacterial strain, designated 4M15T, was isolated from cotton-waste composts used as mushroom cultivation in South Korea. Properties of this isolate were studied on the basis of physiological and biochemical characteristics, fatty acid profile, isoprenoid quinone, DNA G+C content and phylogenetic position based on 16S rRNA gene sequence analysis. The strain was found to form a distinct phylogenetic lineage related to the family 'Flexibacteraceae' within the phylum 'Bacteroidetes'. No recognized species showed >85 % 16S rRNA gene sequence similarity to strain 4M15T. The fatty acid profile of strain 4M15(T) included C(16 : 1)omega7c/iso-C(15 : 0) 2-OH (30.5 %), iso-C(15 : 0) (24.2 %), iso-C(15 : 0) 2-OH/C(16 : 1)omega7c (15.9), iso-C(17 : 0) 3-OH (10.5 %) and C(16 : 0) (5.6 %). The major isoprenoid quinone was menaquinone MK-7. The DNA G+C content was 33.0 mol%. Cells were Gram-negative, strictly aerobic, rod-shaped, non-motile, catalase-positive, oxidase-positive and flexirubin-positive. The strain hydrolysed aesculin, gelatin, starch and tyrosine. Several phenotypic tests could be used to differentiate strain 4M15T from other members of the family 'Flexibacteraceae'. On the basis of the data presented, strain 4M15T should be assigned to the phylum 'Bacteroidetes' as a novel genus and species, for which the name Leadbetterella byssophila gen. nov., sp. nov. is proposed. The type strain is 4M15T (=KACC 11308T=DSM 17132T).
从韩国用作蘑菇栽培的棉籽壳堆肥中分离出一株细菌菌株，命名为4M15T。基于生理生化特性、脂肪酸谱、类异戊二烯醌、DNA G+C含量以及基于16S rRNA基因序列分析的系统发育位置，对该分离株的特性进行了研究。该菌株在拟杆菌门内形成了一个与“柔杆菌科”相关的独特系统发育谱系。没有公认的物种与菌株4M15T的16S rRNA基因序列相似性超过85%。菌株4M15(T)的脂肪酸谱包括C(16 : 1)ω7c/异-C(15 : 0) 2-OH（30.5%）、异-C(15 : 0)（24.2%）、异-C(15 : 0) 2-OH/C(16 : 1)ω7c（15.9）、异-C(17 : 0) 3-OH（10.5%）和C(16 : 0)（5.6%）。主要类异戊二烯醌是甲基萘醌MK-7。DNA G+C含量为33.0 mol%。细胞为革兰氏阴性、严格需氧、杆状、不运动、过氧化氢酶阳性、氧化酶阳性且灵菌红素阳性。该菌株能水解七叶苷、明胶、淀粉和酪氨酸。几种表型测试可用于将菌株4M15T与“柔杆菌科”的其他成员区分开来。根据所提供的数据，菌株4M15T应作为一个新的属和种归入拟杆菌门，为此提出新属名Leadbetterella byssophila gen. nov., sp. nov.。模式菌株为4M15T（=KACC 11308T=DSM 17132T）。
