Department of Microbiology, Eötvös Loránd University, H-1117 Budapest, Pázmány P. stny. 1/C, Hungary.
DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, D-38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2010 Nov;60(Pt 11):2567-2571. doi: 10.1099/ijs.0.019398-0. Epub 2009 Dec 11.
A Gram-negative bacterium, designated P2(T), was isolated from the biofilm developed on the inner surface of an ultrapure cooling water system in a Hungarian power plant and was characterized by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain P2(T) was affiliated with the family 'Flexibacteraceae' in the phylum Bacteroidetes. Its closest relative was Flectobacillus lacus CL-GP79(T) (88.7 % 16S rRNA gene sequence similarity) followed by Arcicella rosea TW5(T) (86.5 %), Arcicella aquatica NO-502(T) (86.4 %), Flectobacillus roseus GFA-11(T) (86.3 %) and Flectobacillus major DSM 103(T) (85.4 %). Cells of strain P2(T) were facultatively anaerobic, non-motile rods. The major fatty acids were C(16 : 1)ω5c (42.5 %), iso-C(15 : 0) 2-OH (17.2 %), iso-C(17 : 0) 3-OH (16.1 %) and iso-C(15 : 0) (8.5 %). The major menaquinone was MK-7 and the predominant polar lipid was phosphatidylethanolamine. The DNA G+C content was 54.5 mol%. Thus, the phenotypic and genotypic analyses clearly showed that strain P2(T) is considerably different from members of other genera in the family 'Flexibacteraceae'. Based on these results, it is concluded that strain P2(T) represents a novel species in a new genus, for which the name Siphonobacter aquaeclarae gen. nov., sp. nov. is proposed, with type strain P2(T) (=DSM 21668(T) =NCAIM B 02328(T)).
一株革兰氏阴性细菌，被命名为 P2(T)，从匈牙利一家发电厂超纯水冷却系统内表面形成的生物膜中分离出来，并通过多相分析进行了表征。基于 16S rRNA 基因序列的系统发育分析表明，菌株 P2(T)属于拟杆菌门的'Flexibacteraceae'科。其最接近的亲缘关系是 Flectobacillus lacus CL-GP79(T)（88.7% 16S rRNA 基因序列相似性），其次是 Arcicella rosea TW5(T)（86.5%）、Arcicella aquatica NO-502(T)（86.4%）、Flectobacillus roseus GFA-11(T)（86.3%）和 Flectobacillus major DSM 103(T)（85.4%）。菌株 P2(T)细胞为兼性厌氧、非运动杆状。主要脂肪酸为 C(16 : 1)ω5c（42.5%）、iso-C(15 : 0) 2-OH（17.2%）、iso-C(17 : 0) 3-OH（16.1%）和 iso-C(15 : 0)（8.5%）。主要的menaquinone 是 MK-7，主要的极性脂是磷脂酰乙醇胺。DNA G+C 含量为 54.5 mol%。因此，表型和基因型分析清楚地表明，菌株 P2(T)与 Flexibacteraceae 科的其他属成员有很大的不同。基于这些结果，可以得出结论，菌株 P2(T)代表一个新属的新型物种，建议使用新属名 Siphonobacter aquaeclarae gen. nov.，sp. nov.，其模式菌株为 P2(T)（=DSM 21668(T)=NCAIM B 02328(T)）。
