Saha P, Chakrabarti T
Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India.
Int J Syst Evol Microbiol. 2006 May;56(Pt 5):991-995. doi: 10.1099/ijs.0.64086-0.
An aquatic bacterium, strain GPTSA100-15T, was isolated on nutritionally poor medium TSBA100 (tryptic soy broth diluted 100 times and solidified with 1.5 % agarose) and characterized using a polyphasic approach. The isolate was unable to grow on commonly used nutritionally rich media such as tryptic soy agar, nutrient agar and Luria-Bertani agar. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was affiliated with the family 'Flexibacteraceae' in the phylum Bacteroidetes. Phylogenetically, it showed closest similarity (94.0 %) with an uncultured bacterial clone, HP1A92, detected in a sludge microbial community. Among the culturable bacteria, the isolate had highest 16S rRNA gene sequence similarity with Leadbetterella byssophila 4M15T (87.8 %). Sequence similarities with other members of the phylum Bacteroidetes were less than 85 %. The fatty acid profile of the isolate grown on TSBA100 indicated that the major fatty acid was iso-C15:0, which is also present in many members of the family 'Flexibacteraceae'. Cells of strain GPTSA100-15T are Gram-negative, strictly aerobic rods. The DNA G+C content of the isolate is 36.9 mol%. Results of phenotypic, chemotaxonomic and phylogenetic analyses clearly indicate that strain GPTSA100-15T represents a new genus within the family 'Flexibacteraceae'; the name Emticicia gen. nov. is proposed for the genus, with Emticicia oligotrophica sp. nov. as the type species. The type strain of Emticicia oligotrophica is GPTSA100-15T (=MTCC 6937T=DSM 17448T).
从营养贫瘠的TSBA100培养基（胰蛋白胨大豆肉汤稀释100倍并用1.5%琼脂糖固化）中分离出一株水生细菌，菌株GPTSA100 - 15T，并采用多相分类法对其进行了鉴定。该分离株无法在常用的营养丰富的培养基上生长，如胰蛋白胨大豆琼脂、营养琼脂和Luria - Bertani琼脂。基于16S rRNA基因序列的系统发育分析表明，该分离株属于拟杆菌门中的“Flexibacteraceae”科。在系统发育上，它与在污泥微生物群落中检测到的未培养细菌克隆HP1A92相似度最高（94.0%）。在可培养细菌中，该分离株与嗜藻莱德杆菌4M15T的16S rRNA基因序列相似度最高（87.8%）。与拟杆菌门其他成员的序列相似度低于85%。在TSBA100上生长的该分离株的脂肪酸谱表明，主要脂肪酸为异C15:0，这在“Flexibacteraceae”科的许多成员中也存在。菌株GPTSA100 - 15T的细胞为革兰氏阴性、严格需氧的杆菌。该分离株的DNA G + C含量为36.9 mol%。表型、化学分类和系统发育分析结果清楚地表明，菌株GPTSA100 - 15T代表“Flexibacteraceae”科内的一个新属；提议将该属命名为Emticicia gen. nov.，将嗜寡营养埃姆蒂西亚菌（Emticicia oligotrophica sp. nov.）作为模式种。嗜寡营养埃姆蒂西亚菌的模式菌株为GPTSA100 - 15T（=MTCC 6937T = DSM 17448T）。
