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酿酒酵母中孢子数量的控制与繁殖：自组织系统的关键作用

Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system.

作者信息

Taxis Christof, Keller Philipp, Kavagiou Zaharoula, Jensen Lars Juhl, Colombelli Julien, Bork Peer, Stelzer Ernst H K, Knop Michael

机构信息

The European Molecular Biology Laboratory, D-69117 Heidelberg, Germany.

出版信息

J Cell Biol. 2005 Nov 21;171(4):627-40. doi: 10.1083/jcb.200507168. Epub 2005 Nov 14.

DOI:10.1083/jcb.200507168
PMID:16286509
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2171547/
Abstract

Spindle pole bodies (SPBs) provide a structural basis for genome inheritance and spore formation during meiosis in yeast. Upon carbon source limitation during sporulation, the number of haploid spores formed per cell is reduced. We show that precise spore number control (SNC) fulfills two functions. SNC maximizes the production of spores (1-4) that are formed by a single cell. This is regulated by the concentration of three structural meiotic SPB components, which is dependent on available amounts of carbon source. Using experiments and computer simulation, we show that the molecular mechanism relies on a self-organizing system, which is able to generate particular patterns (different numbers of spores) in dependency on one single stimulus (gradually increasing amounts of SPB constituents). We also show that SNC enhances intratetrad mating, whereby maximal amounts of germinated spores are able to return to a diploid lifestyle without intermediary mitotic division. This is beneficial for the immediate fitness of the population of postmeiotic cells.

摘要

纺锤体极体（SPB）为酵母减数分裂过程中的基因组遗传和孢子形成提供了结构基础。在孢子形成过程中，当碳源受到限制时，每个细胞形成的单倍体孢子数量会减少。我们发现精确的孢子数量控制（SNC）具有两种功能。SNC可使单个细胞形成的孢子（1 - 4个）产量最大化。这是由三种减数分裂SPB结构成分的浓度调节的，而该浓度取决于可用碳源的量。通过实验和计算机模拟，我们表明分子机制依赖于一个自组织系统，该系统能够根据单一刺激（逐渐增加的SPB成分数量）产生特定模式（不同数量的孢子）。我们还表明，SNC增强了四分体内部的交配，由此最大数量的萌发孢子能够在没有中间有丝分裂的情况下恢复到二倍体生活方式。这对减数分裂后细胞群体的即时适应性有益。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ff1/2171547/860f12d11f28/200507168f9.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ff1/2171547/7b4f40815807/200507168f5.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ff1/2171547/860f12d11f28/200507168f9.jpg

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本文引用的文献

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