Bridges A B, Fisher T C, Scott N, McLaren M, Belch J J
Department of Medicine, University of Dundee, Ninewells Hospital and Medical School, Scotland.
Free Radic Res Commun. 1992;16(2):89-97. doi: 10.3109/10715769209049162.
Previous studies have demonstrated circadian rhythms in the onset of thrombotic events, which occur most commonly in the morning, and also in the fibrinolytic activity of the blood which has a peak in the evening and a trough in the morning. There has recently been increasing interest in the role of white blood cells (WBCs) and free radicals (FRs) in thrombosis. No one has yet investigated the potential circadian variation of WBC aggregation and FRs in humans. We studied the circadian rhythm of WBC aggregation and FR status in 10 healthy male volunteers. Six blood samples were collected at 4 hourly intervals from 12:00 (mid-day) until 08:00 the following morning. The volunteers carried out normal daily activities until 00:00 at which time they went to bed and they remained in bed until 08:00. The following were measured on each sample: WBC aggregation; thiobarbituric acid reactive substances (TBARs), plasma thiols (PSH), red cell lysate thiols (LSH), glutathione (GSH) and superoxide dismutase (SOD) which are all altered in the presence of FR activity. The following parameters demonstrated significant circadian rhythms, WBC aggregation p less than 0.001, TBARs p less than 0.015, PSH p less than 0.001, LSH p less than 0.002. WBC aggregation was lowest at 09:00 and highest at 00:00-04:00. TBARs and PSH both had a peak at 16:00 and a trough at 04:00. LSH had a peak at 12:00 and a trough at 08:00. As the behaviour of WBCs and FR status influence the flow properties of blood, a circadian rhythm in WBC function and FR status may contribute to the time of onset of thrombotic diseases. Moreover, with many studies being currently undertaken in this area, our work indicates the need to standardize sample times.
以往的研究表明，血栓形成事件的发生存在昼夜节律，最常发生在早晨，血液的纤溶活性也存在昼夜节律，其在傍晚达到峰值，在早晨出现低谷。最近，白细胞（WBCs）和自由基（FRs）在血栓形成中的作用越来越受到关注。尚未有人研究过人类白细胞聚集和自由基的潜在昼夜变化。我们研究了10名健康男性志愿者白细胞聚集和自由基状态的昼夜节律。从中午12:00开始，每隔4小时采集一次血样，直至次日上午08:00。志愿者在00:00之前进行正常的日常活动，然后上床睡觉，一直睡到08:00。对每个样本进行以下检测：白细胞聚集；硫代巴比妥酸反应性物质（TBARs）、血浆硫醇（PSH）、红细胞裂解物硫醇（LSH）、谷胱甘肽（GSH）和超氧化物歧化酶（SOD），这些在自由基活性存在时都会发生改变。以下参数表现出显著的昼夜节律，白细胞聚集p<0.001，TBARs p<0.015，PSH p<0.001，LSH p<0.002。白细胞聚集在09:00时最低，在00:00 - 04:00时最高。TBARs和PSH在16:00时均达到峰值，在04:00时出现低谷。LSH在12:00时达到峰值，在08:00时出现低谷。由于白细胞的行为和自由基状态会影响血液的流动特性，白细胞功能和自由基状态的昼夜节律可能与血栓性疾病的发病时间有关。此外，鉴于目前该领域正在进行许多研究，我们的工作表明需要对采样时间进行标准化。
