Bridges A B, Scott N A, Parry G J, Belch J J
University Department of Medicine, Ninewells Hospital and Medical School, Dundee, Scotland, United Kingdom.
Eur J Med. 1993 Apr;2(4):205-8.
To determine the effect of age, sex and smoking on free radical (FR) indices. There is currently considerable research interest into the role of FRs in the pathogenesis of many diseases and it is therefore important to identify factors in healthy subjects which influence the markers used to assess FR activity.
Blood samples were obtained from 123 healthy subjects whose age, sex and smoking habits were recorded. The following were measured on each sample: malondialdehyde (MDA) which is a product of FR mediated lipid peroxidation and the FR scavengers plasma thiols (PSH), red cell glutathione (GSH) and superoxide dismutase (SOD).
Malondialdehyde levels were significantly increased in cigarette smokers (p < 0.02) but no significant age related increase in MDA was detected for the group as a whole. However, female smokers had the highest rate of MDA increase with age followed by male smokers and female non-smokers. MDA levels of male non-smokers were unaffected by age. A significant negative correlation was detected between PSH and age (p < 0.001). GSH was significantly higher in females (p < 0.05) and the red cell content of GSH and SOD were significantly inversely related (p < 0.01).
This study of normal subjects describes an age related increase in malondialdehyde levels, the rate of which is influenced by sex and smoking status. In humans, plasma thiol levels decrease with increasing age, females have higher red cell glutathione and there is a significant inverse relationship between red blood cell superoxide dismutase and glutathione levels. Furthermore, cigarette smoking increases plasma malondialdehyde levels. With the considerable current interest in free radicals, it is important to identify factors which influence the indices used to assess free radical activity in disease states.
确定年龄、性别和吸烟对自由基（FR）指标的影响。目前，对于自由基在多种疾病发病机制中的作用存在大量研究兴趣，因此，识别健康受试者中影响用于评估FR活性标志物的因素非常重要。
从123名健康受试者采集血样，记录其年龄、性别和吸烟习惯。对每个样本检测以下指标：丙二醛（MDA），它是FR介导的脂质过氧化产物；以及FR清除剂血浆硫醇（PSH）、红细胞谷胱甘肽（GSH）和超氧化物歧化酶（SOD）。
吸烟者的丙二醛水平显著升高（p < 0.02），但总体上未检测到丙二醛随年龄有显著增加。然而，女性吸烟者丙二醛随年龄增加的速率最高，其次是男性吸烟者和女性非吸烟者。男性非吸烟者的丙二醛水平不受年龄影响。检测到PSH与年龄之间存在显著负相关（p < 0.001）。女性的GSH显著更高（p < 0.05），且GSH和SOD的红细胞含量显著负相关（p < 0.01）。
这项对正常受试者的研究描述了丙二醛水平随年龄增加，其速率受性别和吸烟状况影响。在人类中，血浆硫醇水平随年龄增长而降低，女性红细胞谷胱甘肽更高，且红细胞超氧化物歧化酶与谷胱甘肽水平之间存在显著负相关。此外，吸烟会增加血浆丙二醛水平。鉴于目前对自由基的广泛关注，识别影响疾病状态下用于评估自由基活性指标的因素很重要。
